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  4. Glypican-3/GPC3 Protein, Mouse (sf9, His)

Glypican-3/GPC3 Protein, Mouse (sf9, His)

Cat. No.: HY-P73078
Handling Instructions

GPC3 is a cell surface proteoglycan that regulates Hedgehog and Wnt signaling. Glypican-3/GPC3 Protein, Mouse (sf9, His) is the recombinant mouse-derived Glypican-3/GPC3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Glypican-3/GPC3 Protein, Mouse (sf9, His) is 558 a.a., with molecular weight of ~62 kDa.

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Description

GPC3 is a cell surface proteoglycan that regulates Hedgehog and Wnt signaling. Glypican-3/GPC3 Protein, Mouse (sf9, His) is the recombinant mouse-derived Glypican-3/GPC3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Glypican-3/GPC3 Protein, Mouse (sf9, His) is 558 a.a., with molecular weight of ~62 kDa.

Background

Glypican-3 (GPC3) Protein, a cell surface proteoglycan, intricately regulates signaling pathways crucial for developmental processes. It negatively modulates the hedgehog signaling pathway by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins, leading to complex internalization and subsequent lysosomal degradation. Conversely, GPC3 exerts positive regulation on both canonical and non-canonical Wnt signaling pathways. In the canonical Wnt pathway, it binds to the Wnt receptor Frizzled, enhancing the interaction between Frizzled and Wnt ligands. GPC3 also binds to CD81, reducing the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Additionally, GPC3 inhibits the dipeptidyl peptidase activity of DPP4. Functionally, GPC3 plays essential roles in limb patterning, skeletal development, renal branching morphogenesis, coronary vascular development, and cell movements during gastrulation. This multifaceted protein exists as a heterodimer formed by disulfide linkage and interacts with various molecules, including DPP4, FGF2, WNT5A, WNT3A, WNT7B, hedgehog proteins SHH and IHH, and Wnt receptors FZD4, FZD7, and FZD8, highlighting its central role in developmental processes.

Species

Mouse

Source

Sf9 insect cells

Tag

C-His

Accession

Q8CFZ4 (M1-P558)

Gene ID

14734  [NCBI]

Molecular Construction
N-term
GPC3 (M1-P558)
Accession # Q8CFZ4
His
C-term
Synonyms
Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5
AA Sequence

MAGTVRTACLLVAMLLGLGCLGQAQPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQMMNPGLPESVLDINECLRGARRDLKVFGSFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNGGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKILKVAHVEHEETLSSRRRELIQKLKSFINFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGKVLDKSLDEEGLESGDCGDDEDECIGSSGDGMVKVKNQLRFLAELAYDLDVDDAPGNKQHGNQKDNEITTSHSVGNMP

Molecular Weight

Approximately 62 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Glypican-3/GPC3 Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Glypican-3/GPC3 Protein, Mouse (sf9, His)
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HY-P73078
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