IgJ Protein, Human (His)
Based on 1 Customer Validation
The IgJ protein links IgM or IgA monomers, nucleating IgM pentamers and inducing IgA dimer or polymer formation. It also binds these immunoglobulins to the secretory component. As part of the sIgA complex, IgJ integrates with IgA monomers, the J chain, and the secretory component (PIGR proteolytic product). IgJ Protein, Human (His) is the recombinant human-derived IgJ protein, expressed by E. coli , with C-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The IgJ protein links IgM or IgA monomers, nucleating IgM pentamers and inducing IgA dimer or polymer formation. It also binds these immunoglobulins to the secretory component. As part of the sIgA complex, IgJ integrates with IgA monomers, the J chain, and the secretory component (PIGR proteolytic product). IgJ Protein, Human (His) is the recombinant human-derived IgJ protein, expressed by E. coli , with C-His labeled tag.
The IgJ protein plays a pivotal role in connecting two monomer units of either IgM or IgA. In the context of IgM, the J chain-joined dimer acts as a nucleating unit for the IgM pentamer, while in the case of IgA, it induces the formation of dimers and/or larger polymers. Additionally, IgJ facilitates the binding of these immunoglobulins to the secretory component. It is an integral part of the secretory IgA (sIgA) complex, which comprises two, four, or five IgA monomers, and two additional non-Ig polypeptides, namely the J chain and the secretory component (the proteolytic product of PIGR).
Technical Parameters
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Species Human
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Source E. coli
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Tag C-His
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Accession
P01591/NP_653247.1 (Q23-D159)
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Molecular Construction
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N-term
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IgJ (Q23-D159)
Accession # P01591/NP_653247.1 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
JCHAIN; JCH; Prev. IGJ; IgJ Chain; IGCJ; J Chain; Immunoglobulin J Chain; Joining Chain Of Multimeric IgA And IgM; Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin Alpha And Mu Polypeptides
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AA Sequence
QEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD
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Molecular Weight
Approximately 26 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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