1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-2
  5. IL-2 Protein, Human (HEK293, His)

IL-2 Protein, Human (HEK293, His)

Cat. No.: HY-P70758
COA Handling Instructions

IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Human (HEK293, His) is the recombinant human-derived IL-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IL-2 Protein, Human (HEK293, His) is 133 a.a., with molecular weight of 14-18 kDa.

For research use only. We do not sell to patients.

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Description

IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Human (HEK293, His) is the recombinant human-derived IL-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IL-2 Protein, Human (HEK293, His) is 133 a.a., with molecular weight of 14-18 kDa.

Background

The cytokine interleukin-2 (IL-2), produced primarily by activated CD4-positive helper T-cells and, to a lesser extent, by activated CD8-positive T-cells and natural killer (NK) cells, plays pivotal roles in the immune response and tolerance. IL-2 binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). This interaction induces oligomerization and conformational changes in the IL-2R subunits, initiating downstream signaling with the phosphorylation of JAK1 and JAK3. Subsequently, JAK1 and JAK3 phosphorylate the receptor, creating a docking site for the phosphorylation of various substrates, including STAT5. This process activates multiple pathways, including STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK pathways. IL-2 functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and promotes robust proliferation of activated B-cells, leading to increased immunoglobulin production. Furthermore, IL-2 plays a crucial role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, essential for maintaining immune tolerance. Additionally, IL-2 participates in the differentiation and homeostasis of various effector T-cell subsets, including Th1, Th2, Th17, as well as memory CD8-positive T-cells.

Biological Activity

The cell proliferation assay using CTLL-2 mouse cytotoxic T cells has a specific activity of ≥1×107 IU/mg.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P60568 (A21-T153)

Gene ID
Synonyms
Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2
AA Sequence

APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

Molecular Weight

14-18 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

IL-2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-2 Protein, Human (HEK293, His)
Cat. No.:
HY-P70758
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