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  6. Phytohemagglutinin

Phytohemagglutinin  (Synonyms: PHA-M)

Cat. No.: HY-N7038 Purity: ≥80.0%
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Phytohemagglutinin (PHA-M), the major seed lectin of the common bean, Phaseolus vulgaris, accumulates in the parenchyma cells of the cotyledons. Phytohemagglutinin is a T-cell activator. Stimulation of human mononuclear leukocytes by Phytohemagglutinin induces the expression of ChAT mRNA, and potentiated ACh synthesis.

For research use only. We do not sell to patients.

Phytohemagglutinin Chemical Structure

Phytohemagglutinin Chemical Structure

CAS No. : 9008-97-3

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10 mg USD 85 In-stock
50 mg USD 255 In-stock
100 mg USD 385 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Phytohemagglutinin:

Phytohemagglutinin Related Antibodies

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Description

Phytohemagglutinin (PHA-M), the major seed lectin of the common bean, Phaseolus vulgaris, accumulates in the parenchyma cells of the cotyledons. Phytohemagglutinin is a T-cell activator. Stimulation of human mononuclear leukocytes by Phytohemagglutinin induces the expression of ChAT mRNA, and potentiated ACh synthesis[1].

IC50 & Target

Apoptosis[1]
In Vitro

Phytohemagglutinin (PHA-M) binds to the membranes of T-cells, stimulates metabolic activity, cell division, and involves inflammatory pathways[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phytohemagglutinin Related Antibodies

Apoptosis Analysis[1]

Cell Line: A20 (mouse B cell lymphoma, TLB-208), BW5147.3 (mouse T cell lymphoma, TIB-47), EL4 (mouse T cell lymphoma, TIB-39)[1]
Concentration: 20 μg/ml
Incubation Time: 20-48 hours
Result: Enhanced apoptosis in A20, BW5147.3 and EL4 cells.
In Vivo

Phytohemagglutinin (100 μg; i.p.; daily for 2 days) inhibits A20 tumor growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CB17-SCID Mice (bearing A20 cells)[1]
Dosage: 100 μg
Administration: I.p.; daily for 2 days
Result: Inhibited A20 tumor growth.
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[Phytohemagglutinin]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 20 mg/mL (Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (Infinity mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥80.0%

COA (241 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phytohemagglutinin9008-97-3PHA-MOthersT-cellB-celldivisioncancerinflammatorylymphoidtumorInhibitorinhibitorinhibit

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