1. Recombinant Proteins
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-5 Receptor
  5. IL-5 Receptor α
  6. IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi)

IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78162
Handling Instructions

IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells and is a transmembrane protein (F343-C362) with His-Avi tags.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock
100 μg $900 Ask For Quote & Lead Time

* Please select Quantity before adding items.

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells and is a transmembrane protein (F343-C362) with His-Avi tags[1][3].

Background

IL-5R alpha is a type I cytokine receptor consisting of two distinct polypeptide chains, alpha and beta. alpha chain is a membrane-penetrating glycoprotein that specifically binds IL-5 and beta chain converts low affinity IL-5R to high affinity IL-5R, forming a heterodimeric receptor complex[1].
IL-5R alpha can induce the activation of multiple intracellular signalling pathways, including JAK/STAT, RAF/MAP, Ras-Raf-ERK and phosphatidylinositol 3-kinase when its binds with IL-5[2].
IL-5R alpha is expressed on eosinophils, basophils and B cells and affects cell survival, apoptosis, differentiation and chemotaxis[3].

Biological Activity
Measured by its binding ability in a functional ELISA. When immobilized Human IL-5 His at 0.5 μg/mL (100μL/Well) , can bind biotinylated Human IL-5 R alpha His and the EC50 is 0.12 ug/mL.
Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q01344 (D21-E335)

Gene ID
Synonyms
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
Molecular Weight

Approximately 38.7 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78162
Quantity:
MCE Japan Authorized Agent: