IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells with His-Avi tags.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells with His-Avi tags[1][3].
Background
IL-5R alpha is a type I cytokine receptor consisting of two distinct polypeptide chains, alpha and beta. alpha chain is a membrane-penetrating glycoprotein that specifically binds IL-5 and beta chain converts low affinity IL-5R to high affinity IL-5R, forming a heterodimeric receptor complex[1].
IL-5R alpha can induce the activation of multiple intracellular signalling pathways, including JAK/STAT, RAF/MAP, Ras-Raf-ERK and phosphatidylinositol 3-kinase when its binds with IL-5[2].
IL-5R alpha is expressed on eosinophils, basophils and B cells and affects cell survival, apoptosis, differentiation and chemotaxis[3].
Verified Bioactivity
1. Measured by its binding ability in a functional ELISA. When immobilized Human IL-5 His at 0.5 μg/mL (100μL/Well) , can bind biotinylated Human IL-5 R alpha His and the EC50 is 0.12 ug/mL.
2. Immobilized Human IL-5, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of ≤0.16μg/ml determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q01344-1 (D21-E335)
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Molecular Construction
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N-term
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IL-5Rα (D21-E335)
Accession # Q01344-1 -
8*His-Avi
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C-term
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Protein Length
Partial Extracellular Domain
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Conjugation
Biotin
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Synonyms
IL5RA; IL-5R Subunit Alpha; Prev. IL5R; Interleukin 5 Receptor Alpha Transcript Variant 7; CDw125; Interleukin-5 Receptor Alpha Chain; Interleukin-5 Receptor Subunit Alpha; Interleukin 5 Receptor Type 3; CD125; IL-5R-Alpha; Interleukin 5 Receptor, Alpha;
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AA Sequence
DLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGNDE
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Predicted Molecular Mass
38.7 kDa
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Molecular Weight
Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5-8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. S Takaki, et al. A critical cytoplasmic domain of the interleukin-5 (IL-5) receptor alpha chain and its function in IL-5-mediated growth signal transduction. Mol Cell Biol. 1994 Nov;14(11):7404-14. [Content Brief]
[2]. R P de Groot, et al. Regulation of proliferation, differentiation and survival by the IL-3/IL-5/GM-CSF receptor family. Cell Signal. 1998 Oct;10(9):619-29. [Content Brief]
[3]. S C Barry, et al. Analysis of interleukin 5 receptors on murine eosinophils: a comparison with receptors on B13 cells. Cytokine. 1991 Jul;3(4):339-45. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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