MGAT2 Protein, Human (HEK293, His)
The MGAT2 protein plays an important role in protein N-glycosylation, catalyzing the transfer of GlcNAc to the free terminal mannose in nascent N-linked glycan chains. This forms a second branch of the complex glycan and is critical for glycoprotein maturation and functional diversity. MGAT2 Protein, Human (HEK293, His) is the recombinant human-derived MGAT2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The MGAT2 protein plays an important role in protein N-glycosylation, catalyzing the transfer of GlcNAc to the free terminal mannose in nascent N-linked glycan chains. This forms a second branch of the complex glycan and is critical for glycoprotein maturation and functional diversity. MGAT2 Protein, Human (HEK293, His) is the recombinant human-derived MGAT2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The MGAT2 protein assumes a fundamental role in protein N-glycosylation, facilitating a crucial step in the modification of nascent glycan chains. Specifically, MGAT2 catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety within the core structure of the nascent N-linked glycan chain. This enzymatic activity results in the formation of the second branch in complex glycans, a process essential for the proper maturation and functional diversity of glycoproteins. By mediating this glycosylation step, MGAT2 plays a central role in the intricate machinery governing the modification of proteins, contributing to cellular processes and functions crucial for overall biological activity.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-6*His
-
Accession
Q10469 (R30-Q447)
-
Molecular Construction
-
N-term
-
MGAT2 (R30-Q447)
Accession # Q10469 -
6*His
-
C-term
-
-
Protein Length
Lumenal Domain
-
Synonyms
MGAT2; GlcNAc-T II; Alpha-1,6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase; UDP-N-Acetylglucosamine:Alpha-6-D-Mannoside Beta-1,2-N-Acetylglucosaminyltransferase II; GNT-II; GLCNACTII; Mannosyl (Alpha-1,6-)-Glycoprotein Beta-1,2-N-Acetylglu
-
AA Sequence
RQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ
-
Predicted Molecular Mass
49.3 kDa
-
Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
