MGAT2 Protein, Human (HEK293, His)
The MGAT2 protein plays an important role in protein N-glycosylation, catalyzing the transfer of GlcNAc to the free terminal mannose in nascent N-linked glycan chains. This forms a second branch of the complex glycan and is critical for glycoprotein maturation and functional diversity. MGAT2 Protein, Human (HEK293, His) is the recombinant human-derived MGAT2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The MGAT2 protein plays an important role in protein N-glycosylation, catalyzing the transfer of GlcNAc to the free terminal mannose in nascent N-linked glycan chains. This forms a second branch of the complex glycan and is critical for glycoprotein maturation and functional diversity. MGAT2 Protein, Human (HEK293, His) is the recombinant human-derived MGAT2 protein, expressed by HEK293 , with C-6*His labeled tag.
The MGAT2 protein assumes a fundamental role in protein N-glycosylation, facilitating a crucial step in the modification of nascent glycan chains. Specifically, MGAT2 catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety within the core structure of the nascent N-linked glycan chain. This enzymatic activity results in the formation of the second branch in complex glycans, a process essential for the proper maturation and functional diversity of glycoproteins. By mediating this glycosylation step, MGAT2 plays a central role in the intricate machinery governing the modification of proteins, contributing to cellular processes and functions crucial for overall biological activity.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q10469 (R30-Q447)
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Molecular Construction
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N-term
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MGAT2 (R30-Q447)
Accession # Q10469 -
6*His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
rHuAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase/MGAT2, His ; Alpha-1; 6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase; Beta-1; 2-N-acetylglucosaminyltransferase II; GlcNAc-T II; NT-II; Mannoside Acetylglucosaminyltransferase 2; N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase II; MGAT2
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AA Sequence
RQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ
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Predicted Molecular Mass
49.3 kDa
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 .
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
N/A
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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