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NAD(P)H-flavin reductase Protein, E.coli (His)

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The NAD(P)H-flavin reductase protein plays a key role in cellular redox processes by catalyzing the reduction of soluble flavins using reduced pyridine nucleotides. This enzyme activity reflects its ability to transfer electrons from NAD(P)H to flavin, participating in important intracellular redox reactions. NAD (P) H-flavin reductase Protein, E.coli (His) is the recombinant E. coli-derived NAD(P)H-flavin reductase protein, expressed by E. coli , with N-His labeled tag.

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  • Species: E.coli
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • NAD(P)H-flavin reductase Protein, E.coli (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The NAD(P)H-flavin reductase protein plays a key role in cellular redox processes by catalyzing the reduction of soluble flavins using reduced pyridine nucleotides. This enzyme activity reflects its ability to transfer electrons from NAD(P)H to flavin, participating in important intracellular redox reactions. NAD (P) H-flavin reductase Protein, E.coli (His) is the recombinant E. coli-derived NAD(P)H-flavin reductase protein, expressed by E. coli , with N-His labeled tag.

Background

The NAD(P)H-flavin reductase protein is an enzyme that catalyzes the reduction of soluble flavins using reduced pyridine nucleotides, such as NADH or NADPH. This enzymatic activity is crucial for maintaining cellular redox balance and is involved in various metabolic processes where flavin coenzymes serve as electron carriers. By utilizing reduced pyridine nucleotides, NAD(P)H-flavin reductase plays a key role in transferring electrons to flavins, thereby contributing to the regulation of cellular oxidative stress and the redox state. This enzyme's involvement in flavin reduction underscores its significance in diverse biological pathways and highlights its role in modulating cellular responses to oxidative conditions.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag N-His
  • Accession

    P0AEN1 (T2-I233)

  • Gene ID
    948325  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • FRE_ECOLI (T2-I233)
      Accession # P0AEN1
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    FRZB; FZRB; Frizzled Related Protein; HFIZ; Secreted Frizzled-Related Protein 3; Frezzled; FRZB1; SFRP-3; SFRP3; FrzB-1; FRE; Uncharacterized Protein FRZB; Frizzled-Related Protein 1; Frizzled Homolog-Related; FRZB-PEN; Frizzled-Related Protein; FRZB-1; F

  • AA Sequence

    TTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDERDKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDEERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHPGLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSERNAREDRLFGDAFAFI

  • Predicted Molecular Mass

    30.1 kDa

  • Molecular Weight

    Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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