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PCBP1 Protein, Human (His)

Cat. No.: HY-P74649
COA Handling Instructions

PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag. The total length of PCBP1 Protein, Human (His) is 355 a.a., with molecular weight of ~42 kDa.

For research use only. We do not sell to patients.

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5 μg $68 In-stock
10 μg $115 In-stock
50 μg $320 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag. The total length of PCBP1 Protein, Human (His) is 355 a.a., with molecular weight of ~42 kDa.

Background

PCBP1 is a single-stranded nucleic acid binding protein with a specific affinity for oligo dC. Paired with PCBP2, it is essential for erythropoiesis, potentially influencing mRNA splicing processes. In the context of poliovirus infection, PCBP1 assumes a crucial role in initiating viral RNA replication in collaboration with the viral protein 3CD. This highlights its multifunctional capacity in nucleic acid binding and its involvement in fundamental cellular and viral processes.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q15365 (D2-C356)

Gene ID
Molecular Construction
N-term
His
PCBP1 (D2-C356)
Accession # Q15365
C-term
Synonyms
Poly(rC)-binding protein 1; Alpha-CP1; hnRNP E1; PCBP1
AA Sequence

DAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGC

Molecular Weight

Approximately 42 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 500 mM NaCl, 20% glycerol, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PCBP1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PCBP1 Protein, Human (His)
Cat. No.:
HY-P74649
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