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PCBP1 Protein, Human (His)

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Based on 1 publication(s) in Google Scholar

PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag.

Background

PCBP1 is a single-stranded nucleic acid binding protein with a specific affinity for oligo dC. Paired with PCBP2, it is essential for erythropoiesis, potentially influencing mRNA splicing processes. In the context of poliovirus infection, PCBP1 assumes a crucial role in initiating viral RNA replication in collaboration with the viral protein 3CD. This highlights its multifunctional capacity in nucleic acid binding and its involvement in fundamental cellular and viral processes.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His
  • Accession

    NP_006187.2 (D2-S356)

  • Gene ID
    5093  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • PCBP1 (D2-S356)
      Accession # NP_006187.2
    • C-term
  • Protein Length

    Partial

  • Synonyms

    PCBP1; Nucleic Acid-Binding Protein SUB2.3; Poly(RC) Binding Protein 1; Epididymis Secretory Protein Li 85; Heterogeneous Nuclear Ribonucleoprotein E1; Alpha-CP1; Poly(RC)-Binding Protein 1; HEL-S-85; HnRNP-E1; Heterogenous Nuclear Ribonucleoprotein E1; H

  • AA Sequence

    DAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

  • Predicted Molecular Mass

    38.3 kDa

  • Molecular Weight

    Approximately 42 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 500 mM NaCl, 20% glycerol, pH 8.0 or 50 mM Tris, 500 mM NaCl, 5% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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