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  4. PFKFB1 Protein, Human (HEK293, His)

PFKFB1 Protein, Human (HEK293, His)

Cat. No.: HY-P71118
COA Handling Instructions

PFKFB1 protein orchestrates the synthesis and degradation of fructose 2,6-bisphosphate, regulating cellular energy metabolism. Serving as a key enzyme, PFKFB1 modulates fructose 2,6-bisphosphate levels, impacting glycolysis and gluconeogenesis. Its dual role in both synthesis and breakdown is crucial for maintaining cellular energy homeostasis, ensuring adaptability to changing energy demands and environmental conditions. PFKFB1 Protein, Human (HEK293, His) is the recombinant human-derived PFKFB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PFKFB1 Protein, Human (HEK293, His) is 470 a.a., with molecular weight of ~60.0 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

PFKFB1 protein orchestrates the synthesis and degradation of fructose 2,6-bisphosphate, regulating cellular energy metabolism. Serving as a key enzyme, PFKFB1 modulates fructose 2,6-bisphosphate levels, impacting glycolysis and gluconeogenesis. Its dual role in both synthesis and breakdown is crucial for maintaining cellular energy homeostasis, ensuring adaptability to changing energy demands and environmental conditions. PFKFB1 Protein, Human (HEK293, His) is the recombinant human-derived PFKFB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PFKFB1 Protein, Human (HEK293, His) is 470 a.a., with molecular weight of ~60.0 kDa.

Background

PFKFB1 protein takes center stage in the intricate orchestration of the synthesis and degradation of fructose 2,6-bisphosphate. As a key regulatory enzyme, PFKFB1 plays a crucial role in controlling cellular energy metabolism by modulating the levels of fructose 2,6-bisphosphate. This dual-function protein holds the responsibility of both synthesizing and breaking down fructose 2,6-bisphosphate, a pivotal metabolite that regulates glycolysis and gluconeogenesis. The dynamic interplay orchestrated by PFKFB1 is integral to maintaining the delicate balance of cellular energy homeostasis, influencing key metabolic pathways to ensure the cell's adaptability to varying energy demands and environmental conditions.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P16118 (S2-Y471)

Gene ID
Molecular Construction
N-term
PFKFB1 (S2-Y471)
Accession # P16118
6*His
C-term
Synonyms
6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 1; 6PF-2-K/Fru-2; 6-P2ase liver isozyme; Fructose-2; 6-bisphosphatase; PFKFB1; F6PK; PFRX
AA Sequence

SPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Molecular Weight

Approximately 60.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Trehalose, 1 mM EDTA, pH 7.8.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PFKFB1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PFKFB1 Protein, Human (HEK293, His)
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HY-P71118
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