PSD95 Protein, Rat (His)

PSD95 protein plays a crucial role in PDZ domain binding, enzyme binding and signaling receptor binding. It is a structural component of the postsynaptic density and is involved in the regulation of synaptic transmission and cellular organization. PSD95 Protein, Rat (His) is the recombinant rat-derived PSD95 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PSD95 Protein, Rat (His) is 724 a.a., with molecular weight of 100-110 kDa.

PSD95 Protein plays a crucial role in various functions, including PDZ domain binding, enzyme binding, and signaling receptor binding. It serves as a structural component of the postsynaptic density and is involved in processes such as modulation of chemical synaptic transmission, positive regulation of cellular component organization, and postsynapse organization. It is located in different cellular components, including the dendrite, juxtaparanode region of the axon, and postsynaptic density. PSD95 Protein is active in glutamatergic synapses and serves as an anchored component of the postsynaptic density membrane. It colocalizes with the postsynaptic membrane and is identified as a biomarker in Parkinson's disease and temporal lobe epilepsy. In humans, mutations in the orthologous DLG4 gene have been linked to autosomal dominant non-syndromic intellectual disability. [provided by Alliance of Genome Resources, Apr 2022] PSD95 Protein exhibits biased expression in the brain (RPKM 195.0), adrenal gland (RPKM 29.1), and seven other tissues.

Rat

E. coli

N-6*His

NP_062567 (M1-L724)

29495  [NCBI]

MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

100-110 kDa

Greater than 80% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.