PSMA Protein, Mouse (Biotinylated, HEK293, His)
Based on 1 Customer Validation
The PSMA protein is a multifunctional enzyme with folate hydrolase and NAALADase activities and a preference for tri-alpha-glutamic acid peptides. It absorbs folate in the intestines, aids in important metabolic processes, and modulates excitatory neurotransmission in the brain by hydrolyzing NAAG to release glutamate. PSMA Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived PSMA protein, expressed by HEK293 , with N-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The PSMA protein is a multifunctional enzyme with folate hydrolase and NAALADase activities and a preference for tri-alpha-glutamic acid peptides. It absorbs folate in the intestines, aids in important metabolic processes, and modulates excitatory neurotransmission in the brain by hydrolyzing NAAG to release glutamate. PSMA Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived PSMA protein, expressed by HEK293 , with N-His labeled tag.
Background
PSMA, a multifunctional enzyme, possesses both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activities, with a notable preference for tri-alpha-glutamate peptides. It plays a crucial role in the intestinal uptake of folate, contributing to essential metabolic processes. Within the brain, PSMA acts as a modulator of excitatory neurotransmission by hydrolyzing the neuropeptide N-acetylaspartylglutamate (NAAG), leading to the release of glutamate. Additionally, it demonstrates dipeptidyl-peptidase IV type activity and efficiently cleaves Gly-Pro-AMC in vitro, showcasing its versatile enzymatic functions.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-8*His
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Accession
O35409 (K45-A752)
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Molecular Construction
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N-term
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8*His
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PSMA (K45-A752)
Accession # O35409 -
C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
FOLH1; Folylpoly-Gamma-Glutamate Carboxypeptidase; Prev. FOLH; Membrane Glutamate Carboxypeptidase; NAALAD1; Glutamate Carboxylase II; GCPII; NAALADase I; PSMA; NAALAdase; PSM; FGCP; Glutamate Carboxypeptidase II; MGCP; Glutamate Carboxypeptidase 2; Folate Hydrolase (Prostate-Specific Membrane Antigen) 1; GCP2; N-Acetylated Alpha-Linked Acidic Dipeptidase 1; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Prostate-Specific Membrane Antigen; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folate Hydrolase 1; Cell Growth-Inhibiting Gene 27 Protein
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AA Sequence
KPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA
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Predicted Molecular Mass
80.6 kDa
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Molecular Weight
Approximately 88-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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