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Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains. TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) is a recombinant protein (L30-S390) produced by HEK293 cells with His tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains. TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) is a recombinant protein (L30-S390) produced by HEK293 cells with His tag[1][2].

Background

Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. The pro-protein is enzymatically cleaved to generate an amino-terminal Latency Associated Peptide (LAP), but this product remains non-covalently associated with TGFβ-1 keeping it in a biologically inactive state.

Verified Bioactivity

1. Immobilized Mouse Latent TGF beta 1, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, hFc Tag with the EC50 of 0.39-0.43 μg/mL determined by ELISA.
2. Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is <0.5 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.1173 ng/mL, corresponding to a specific activity is 8.525×106 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag N-His
  • Accession

    P04202 (L30-S390)

  • Gene ID
    21803  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • TGFB1 (L30-S390)
      Accession # P04202
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    TGFB1; Transforming Growth Factor Beta1; Prev. TGFB; Camurati-Engelmann Disease; Prev. DPD1; Latency-Associated Peptide; TGFbeta; TGF-Beta-1; CED; TGF-Beta1; Transforming Growth Factor Beta-1 Proprotein; IBDIMDE; Prepro-Transforming Growth Factor Beta-1;

  • AA Sequence

    LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

  • Predicted Molecular Mass

    42.4 kDa

  • Molecular Weight

    Approximately 13-15 kDa & 40-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. K. Miyazono, et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. The EMBO Journal (1991)10:1091-1101.

[2]. M Selvakumaran, et al. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells. Mol Cell Biol. 1994 Apr;14(4):2352-60. [Content Brief]

[3]. Chambaz E.M., et al. Transforming Growth Factor-βs: A Multifunctional Cytokine Family. Implication in the Regulation of Adrenocortical Cell Endocrine Functions. 1991.

[4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10. [Content Brief]

[5]. Justin Rustenhoven, et al. TGF-beta1 regulates human brain pericyte inflammatory processes involved in neurovasculature function. Justin Rustenhoven. 2016 Feb 11;13:37. [Content Brief]

[6]. Kai Zhang, et al. Essential role of microglial transforming growth factor-β1 in antidepressant actions of (R)-ketamine and the novel antidepressant TGF-β1. Transl Psychiatry. 2020 Jan 27;10(1):32. [Content Brief]

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Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.1173 ng/mL, corresponding to a specific activity is 8.525×106 units/mg.

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 Oct 31;30(10):45355. [Abstract]

    Customer Validation
     Latent TGF beta 1/TGFB1 Protein (TGFβ, 10 ng/mL; 15 min) could induce smooth muscle cell hypertrophy. Scale bar = 100 µm.

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 Oct 31;30(10):45355. [Abstract]

    Customer Validation
     Latent TGF beta 1/TGFB1 Protein (TGFβ, 10 ng/mL; 15 min) treatment significantly upregulated both CTSS and ACTA2 expression in bladder smooth muscle cells.

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jun 12:327:117986. [Abstract]

    Customer Validation
     The viability of DML-treated HK2 cells without and with TGF-β1 (Latent TGF beta 1/TGFB1 Protein, 10 ng/mL; 24 h) stimulation was determined by a CCK-8 assay. (n = 3)

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jun 12:327:117986. [Abstract]

    Customer Validation
     Latent TGF beta 1/TGFB1 Protein (TGF-β1, 10 ng/mL; 24 h) decreased the MMP in HK2 cells. However, these detrimental effects were significantly reversed by DML.

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: iScience. 2023 Feb 28;26(4):106289. [Abstract]

    Customer Validation
     Control and SOX4 knockdown C3H10T1/2 MSCs were treated with vehicle or 5 ng/mL TGFβ1 (Latent TGF beta 1/TGFB1 Protein) until confluency. The expression of SOX4, PPARγ2in17 pre-differentiated cells and adipogenic genes in post-differentiated cells were18 analyzed by Western blotting.

  • 1/16

    Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: iScience. 2023 Feb 28;26(4):106289. [Abstract]

    Customer Validation
     Control and SOX4 knockdown C3H10T1/2 MSCs were treated with vehicle or 5 ng/mL TGFβ1 (Latent TGF beta 1/TGFB1 Protein) until confluency. The expression of SOX4, PPARγ2in17 pre-differentiated cells and adipogenic genes in post-differentiated cells were18 analyzed by qPCR.

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)
  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) Product Image

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 Oct 31;30(10):45355. [Abstract]

    Latent TGF beta 1/TGFB1 Protein (TGFβ, 10 ng/mL; 15 min) could induce smooth muscle cell hypertrophy. Scale bar = 100 µm.

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 Oct 31;30(10):45355. [Abstract]

    Latent TGF beta 1/TGFB1 Protein (TGFβ, 10 ng/mL; 15 min) treatment significantly upregulated both CTSS and ACTA2 expression in bladder smooth muscle cells.

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jun 12:327:117986. [Abstract]

    The viability of DML-treated HK2 cells without and with TGF-β1 (Latent TGF beta 1/TGFB1 Protein, 10 ng/mL; 24 h) stimulation was determined by a CCK-8 assay. (n = 3)

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jun 12:327:117986. [Abstract]

    Latent TGF beta 1/TGFB1 Protein (TGF-β1, 10 ng/mL; 24 h) decreased the MMP in HK2 cells. However, these detrimental effects were significantly reversed by DML.

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: iScience. 2023 Feb 28;26(4):106289. [Abstract]

    Control and SOX4 knockdown C3H10T1/2 MSCs were treated with vehicle or 5 ng/mL TGFβ1 (Latent TGF beta 1/TGFB1 Protein) until confluency. The expression of SOX4, PPARγ2in17 pre-differentiated cells and adipogenic genes in post-differentiated cells were18 analyzed by Western blotting.

  • Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: iScience. 2023 Feb 28;26(4):106289. [Abstract]

    Control and SOX4 knockdown C3H10T1/2 MSCs were treated with vehicle or 5 ng/mL TGFβ1 (Latent TGF beta 1/TGFB1 Protein) until confluency. The expression of SOX4, PPARγ2in17 pre-differentiated cells and adipogenic genes in post-differentiated cells were18 analyzed by qPCR.