SMO-IN-2

製品番号: HY-150564 純度: 98.12%: Data Sheet SDS COA 取扱説明書
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SMO-IN-2 is a potent smoothened (SMO) inhibitor with an IC₅₀ value of 123.4 nM for hedgehog (Hh) signaling pathway. SMO-IN-2 has antiproliferative activity against human medulloblastoma cell line Daoy. Anticancer activity.

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SMO-IN-2 構造式

CAS 番号 : 1822355-27-0

容量	価格（税別）	在庫状況	数量
5 mg	$460	在庫あり	Estimated Time of Arrival: December 31
10 mg	$690	在庫あり	Estimated Time of Arrival: December 31
25 mg	$1120	在庫あり	Estimated Time of Arrival: December 31
50 mg	$1500	在庫あり	Estimated Time of Arrival: December 31
100 mg		お問い合わせ
200 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IC₅₀: 123.4 nM (Hh)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A2058	IC₅₀	> 50 μM Compound: 1	Antiproliferation activity against human A2058 cells incubated for 72 hrs by MTT assay Antiproliferation activity against human A2058 cells incubated for 72 hrs by MTT assay	[PMID: 32063403]
Daoy	IC₅₀	0.51 μM Compound: 1	Antiproliferative activity against human DaOY cells incubated for 72 hrs by MTT assay Antiproliferative activity against human DaOY cells incubated for 72 hrs by MTT assay	[PMID: 32063403]

体外実験

SMO-IN-2 (compound 1) has antiproliferative activity against human medulloblastoma cell line Daoy with an IC₅₀ of 0.51 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

503.49

分子式

C₂₅H₂₅F₄N₅O₂

CAS 番号

1822355-27-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(N(C)C1CCN(C(C2=CC=CC=C2NC3=CC=NN3C)=O)CC1)C4=CC=C(F)C=C4C(F)(F)F

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 10 mg/mL (19.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9861 mL	9.9307 mL	19.8614 mL
5 mM	0.3972 mL	1.9861 mL	3.9723 mL
10 mM	0.1986 mL	0.9931 mL	1.9861 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 98.12%

Select Batch:

データシート (275 KB) SDS (393 KB)

COA (253 KB) HNMR (481 KB) LCMS (96 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Ji D, et al. Design, synthesis and biological evaluation of anthranilamide derivatives as potent SMO inhibitors. Bioorg Med Chem. 2020 Mar 15;28(6):115354. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9861 mL	9.9307 mL	19.8614 mL	49.6534 mL
	5 mM	0.3972 mL	1.9861 mL	3.9723 mL	9.9307 mL
	10 mM	0.1986 mL	0.9931 mL	1.9861 mL	4.9653 mL
	15 mM	0.1324 mL	0.6620 mL	1.3241 mL	3.3102 mL

SMO-IN-2 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMO-IN-21822355-27-0SmoHedgehogSmoothenedanticancermedulloblastomaDaoyInhibitorinhibitorinhibit

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