Robotnikinin
Based on 3 publication(s) in Google Scholar
Robotnikinin is a small molecule capable of binding to and inhibiting the activity of Sonic Hedgehog (Shh) signaling up stream of Smo.
For research use only. We do not sell to patients.
- Purity: 98.98%
- CAS No.: 1132653-79-2
- Formula: C25H27ClN2O4
- Molecular Weight:454.95
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Robotnikinin
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C3H 10T1/2 | IC50 |
>25 μM
Compound: 1
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Inhibition of human Shh-induced mouse C3H10T1/2 cell differentiation after 48 hrs by alkaline phosphatase reporter assay
Inhibition of human Shh-induced mouse C3H10T1/2 cell differentiation after 48 hrs by alkaline phosphatase reporter assay
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[PMID: 23074541] |
| NIH3T3 | EC50 |
4 μM
Compound: 91
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Inhibition of sonic hedgehog pathway in mouse NIH-3T3 cells
Inhibition of sonic hedgehog pathway in mouse NIH-3T3 cells
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[PMID: 21381769] |
| Shh Light II | EC50 |
4 μM
Compound: 17
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Inhibition of SHH in mouse Shh Light2 cells after 30 hrs by GLI-responsive bright glo luciferase reporter gene assay
Inhibition of SHH in mouse Shh Light2 cells after 30 hrs by GLI-responsive bright glo luciferase reporter gene assay
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[PMID: 19819139] |
Robotnikinin demonstrates ShhN-binding capacity at concentrations between 1.56 μM and 25 μM, with a KD of 3.1 μM derived from kinetic data[2].
Robotnikinin (50 µM) prevents the ShhN-induced LC3-II increase[3].
Robotnikinin (5 μM) downregulates classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells, suggesting a link between Hh signaling and the NF-κB pathway in MM[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NCI-H929 or U266 cells.
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Concentration:5 μM.
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Incubation Time:48 h.
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Result:Downregulated classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells.
Chemical Information
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CAS No. 1132653-79-2
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Appearance Solid
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Molecular Weight 454.95
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Formula C25H27ClN2O4
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Color White to off-white
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SMILES
O=C(NCC1=CC=C(Cl)C=C1)C[C@H](C/C=C/CC2)C(NC[C@@H](C3=CC=CC=C3)OC2=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Neural Regen Res
M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats. [Abstract]2023 Oct;18(10):2208-2218. PMID: 37056140 -
Neurochem Int
M2a macrophages regulate fibrosis and affect the outcome after stroke via PU.1/mTOR pathway in fibroblasts. [Abstract]2024 Feb:173:105674. PMID: 38184171 -
Solvent & Solubility
DMSO : 20 mg/mL (43.96 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Manuel Hitzenberger, et al. The Binding Mode of the Sonic Hedgehog Inhibitor Robotnikinin, a Combined Docking and QM/MM MD Study. Front Chem [Content Brief]
[2]. Benjamin Z Stanton, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6. [Content Brief]
[3]. Ronald S Petralia, et al. Sonic hedgehog promotes autophagy in hippocampal neurons. Biol Open. 2013 Apr 8;2(5):499-504. [Content Brief]
[4]. Ke Cai, et al. Targeting the cross-talk between the hedgehog and NF-κB signaling pathways in multiple myeloma. Leuk Lymphoma. 2019 Mar;60(3):772-781. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1980 mL | 10.9902 mL | 21.9804 mL | 54.9511 mL |
| 5 mM | 0.4396 mL | 2.1980 mL | 4.3961 mL | 10.9902 mL | |
| 10 mM | 0.2198 mL | 1.0990 mL | 2.1980 mL | 5.4951 mL | |
| 15 mM | 0.1465 mL | 0.7327 mL | 1.4654 mL | 3.6634 mL | |
| 20 mM | 0.1099 mL | 0.5495 mL | 1.0990 mL | 2.7476 mL | |
| 25 mM | 0.0879 mL | 0.4396 mL | 0.8792 mL | 2.1980 mL | |
| 30 mM | 0.0733 mL | 0.3663 mL | 0.7327 mL | 1.8317 mL | |
| 40 mM | 0.0550 mL | 0.2748 mL | 0.5495 mL | 1.3738 mL |
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