Soporidine
Soporidine is an antagonist of germination of the parasitic plant Striga hermonthica. Soporidine specifically inhibits a S. hermonthica strigolactone receptor and inhibits the parasite's germination. Soporidine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 1060376-43-3
- Formula: C27H30F3NO3
- Molecular Weight:473.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Soporidine binds to the AtHTL receptor and to a key Strigolactone (SL) receptor in S. hermonthica, inhibiting Striga germination in the presence of SLs. Soporidine could serve as the basis for the development of Strigolactone (SL) antagonist to combat Striga infestations. Soporidine is a potent antagonist in GR24-dependent Arabidopsis germination assay and shows no enhancement of SL signaling mutant phenotypes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1060376-43-3
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Molecular Weight 473.53
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Formula C27H30F3NO3
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SMILES
O=C(OCC)C1(CCN(CC1)CC2=CC=C(C=C2)C#CCCO)CC3=CC=CC(C(F)(F)F)=C3
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Synonyms
RG4; SOP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Soporidine Related Classifications
HY-114800 Related Classifications
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