Tetrabromorhodamine 123 bromide

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Cells are incubated with DMEM containing 5 μM TBR and maintained in the dark in a CO₂ incubator at 37°C.
3. After 1 h, chang the medium to normal culture medium without phenol red, and cells were exposed to visible light with a Xe arc with a filter.
B. The intracellular localization of TBR:
1. Cells are cultured in glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol.
2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM).
3. Cells are incubated at 48°C for 20 min and then further incubated at 37°C for 1 h, after which the solution is changed to normal medium containing 5 μM TBR.
4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), and cells were incubated for 15 min.
5. Cells are washed with phosphate-buffered saline (plus Ca²⁺, Mg²⁺) and then observed with the aid of confocal laser scanning microscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.