Transketolase-IN-4
Based on 1 Customer Validation
Transketolase-IN-4 is a potent inhibitor of transketolase with an IC50 value of 3.9 μM. Transketolase-IN-4 inhibits the proliferation of tumor cells including SW620, LS174T and MIA PaCa-2. Transketolase-IN-4 can be used for tumor research.
For research use only. We do not sell to patients.
- Purity : 99.29%
- CAS No.: 419547-73-2
- Formula: C19H14ClN3O
- Molecular Weight:335.79
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Transketolase-IN-4 inhibits the proliferation of tumor cells SW620, LS174T and MIA PaCa-2, with GI50 values of 0.4, 9.3 and 10.4 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 419547-73-2
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Appearance Solid
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Molecular Weight 335.79
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Formula C19H14ClN3O
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Color White to off-white
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SMILES
O=C1C=C(CC2=CC=CC=C2)NC3=C(C4=CC=C(C=C4)Cl)C=NN13
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (372.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9781 mL | 14.8903 mL | 29.7805 mL | 74.4513 mL |
| 5 mM | 0.5956 mL | 2.9781 mL | 5.9561 mL | 14.8903 mL | |
| 10 mM | 0.2978 mL | 1.4890 mL | 2.9781 mL | 7.4451 mL | |
| 15 mM | 0.1985 mL | 0.9927 mL | 1.9854 mL | 4.9634 mL | |
| 20 mM | 0.1489 mL | 0.7445 mL | 1.4890 mL | 3.7226 mL | |
| 25 mM | 0.1191 mL | 0.5956 mL | 1.1912 mL | 2.9781 mL | |
| 30 mM | 0.0993 mL | 0.4963 mL | 0.9927 mL | 2.4817 mL | |
| 40 mM | 0.0745 mL | 0.3723 mL | 0.7445 mL | 1.8613 mL | |
| 50 mM | 0.0596 mL | 0.2978 mL | 0.5956 mL | 1.4890 mL | |
| 60 mM | 0.0496 mL | 0.2482 mL | 0.4963 mL | 1.2409 mL | |
| 80 mM | 0.0372 mL | 0.1861 mL | 0.3723 mL | 0.9306 mL | |
| 100 mM | 0.0298 mL | 0.1489 mL | 0.2978 mL | 0.7445 mL |
Transketolase-IN-4 Related Classifications
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