BD-105

1Guidelines (The following are our recommended protocols; this is a guideline and should be modified according to your specific needs).
1. Stock Solution Preparation
1.1 Solvent: For most dyes, organic solvents such as anhydrous DMSO are typically used for dissolution.
1.2 Concentration Recommendation: Generally, a high concentration of 1-10 mM stock solution is recommended.
1.3 Storage Conditions: Store at -20 ℃ or -80 ℃ protected from light and avoid repeated freeze-thaw cycles.
2. Working Solution Preparation
2.1 Diluent: Serum-free culture medium or PBS is typically used. Proteins and esterases in serum may interfere with staining or cause dye hydrolysis.
2.2 Working Concentration: 0.5-10 μM
Note: Please adjust the dye working solution concentration according to your actual situation and prepare fresh before use.
3. Cell Staining
3.1 Culture cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate excess medium.
3.3 Add working solution, gently shake to completely cover the cells, and incubate at 37 ℃ for 24 hours.
3.4 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope or flow cytometry.
Note: If flow cytometry is required, the cells should be resuspended with trypsin before staining.
BD-105 (500 nM; 24 h) selectively stains glucagon-secreting AlphaTC1 cells, with no detectable staining in BetaTC6 or HeLa cells^[1].
BD-105 (10 μM) in buffer has an absorption maximum of 550 nm and emission maximum of 568 nm with a quantum yield of ~0.012; upon addition of 200 μM glucagon to 1 μM BD-105, quantum yield increases to 0.151 with absorption and emission maxima shifted to 553 nm and 570 nm, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.