Wychimicin A
Wychimicin A is a spirotetronate polyketide, can be isolated from the rare actinomycete Actinocrispum wychmicini strain MI503-AF4. Wychimicin A shows strong antibacterial activity against methicillin-resistant Staphylococcus. aureus (IC50=0.125-0.5 μg/mL) and Enterococcus. faecalis/faecium (IC50=0.125-0.25 μg/mL).
For research use only. We do not sell to patients.
- Formula: C47H60ClNO11
- Molecular Weight:850.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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Molecular Weight 850.43
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Formula C47H60ClNO11
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SMILES
CC1=CC(Cl)=C(C(C(N[C@@H]2[C@H](O[C@H](C[C@@H]2O)O[C@]([H])(C/C=C/C3[C@@]4([C@]5([C@]([H])(C=C3CC)[C@H]([C@H](C[C@@H]5C)O)O)[H])C)/C(C)=C/[C@H]6[C@]7(C[C@@H](C=C6)C)C(O)=C(C(O7)=O)C4=O)C)=O)=C1O)C
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Structure Classification
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Initial Source
Actinocrispum wychmicini
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
Purity & Documentation
References
[1]. Kimura T, et al. Wychimicins, a new class of spirotetronate polyketides from Actinocrispum wychmicini MI503-A4. J Antibiot (Tokyo). 2022 Oct;75(10):535-541. [Content Brief]
[2]. Hatano M, et al. Actinocrispum wychmicini gen. nov., sp. nov., a novel member of the family Pseudonocardiaceae, isolated from soil. Int J Syst Evol Microbiol. 2016 Nov;66(11):4779-4784. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Wychimicin A Related Classifications
HY-N10561 Related Classifications
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