YM-216391
YM-216391 is an antitumor cyclic peptide that can be isolated from the cultured mycelium of Streptomyces nobilis JCM 4274. YM-216391 exhibits potent cytotoxic activity against a variety of human cancer cell lines and can be used in anticancer research.
For research use only. We do not sell to patients.
- Formula: C34H32N8O7S
- Molecular Weight:696.73
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
YM-216391 inhibits the growth of human cervical cancer HeLa S3 cells in a dose-dependent manner with an IC50 value of 14 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 696.73
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Formula C34H32N8O7S
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SMILES
O=C1C2=C(OC(C3=CSC(C4=COC(C5=COC(C6=COC(CNC([C@H](C(C)C)NC([C@@]([H])([C@H](CC)C)N1)=O)=O)=N6)=N5)=N4)=N3)=N2)C7=CC=CC=C7
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Structure Classification
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Initial Source
Streptomyces nobilis
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
YM-216391 Related Classifications
HY-N5208 Related Classifications
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