α2-3,6 Neuraminidase, Bifidobacterium infantis 

α2-3,6 Neuraminidase, Bifidobacterium infantis is a highly specific exoglycosidase that catalyzes the hydrolysis of non-reducing terminal α2-3 and α2-6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. α2-3,6 Neuraminidase does not exhibit activity on α2-8 or branched sialic acids^[1].

others

Product Information
This product is recombinantly expressed in E. coli, with C-terminal His Tag, molecular weight 42 kDa, and storage solution 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol.

Instructions
1. Add substrates to the reaction system in sequence, add 5 mM MnCl2, adjust the pH to 5.0 and mix well, and add appropriate amount of enzyme.
2. Incubate the sample at 37°C for 2-4 hours or overnight.
Note: When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation. Low-speed oscillation mixing can increase the conversion rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-67-6

Liquid

Colorless to light yellow

[a2-3,6 Neuraminidase, Bifidobacterium infantis]

3.2.1.18

>4000 U/mg protein

One unit is defined as the amount of enzyme that hydrolyze of 1 nmol Neu5Aα2, 6 Galβ1, 4Glc to Gal β1, 4Glc per min at 37°C, pH 5.0.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Xilong Wang, et al. A novel strategy for quantification of α2,3- and α2,6-linked sialic acids in sialylated glycoproteins. Carbohydr Res. 2023 Sep:531:108892.  [Content Brief]