β-Mannosidase
β-Mannosidase is a lysosomal exo‑acting enzyme. β-Mannosidase catalyzes hydrolysis of β‑1,4‑linked mannosides, non‑reducing terminal mannoses in N‑glycoproteins, and Man‑β‑1,4‑GlcNAc linkages to release mannose. β-Mannosidase contributes to glycoprotein catabolism and supports Bifidobacteria colonization via N‑glycan depolymerization. β-Mannosidase can be used for the research of β-mannosidosis and β-mannosidase deficiency.
For research use only. We do not sell to patients.
- CAS No.: 9025-43-8
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
β-Mannosidase (10 nM; 1 h) catalyzes the hydrolysis of pNP-Man, with a Km value of 1.42 mM and a kcat value of 72.2 s−1; under in vitro conditions, the activities of disease mutants W466X, R182W, G392E and S505P are significantly reduced or completely lost, while R641H, R638H and L749H retain full wild-type activity[2].
β-Mannosidase activity is undetectable in affected individuals and reduced in carriers, following autosomal recessive inheritance[3].
Purified β-Mannosidase (0.1-50 mM; 5-30 min) derived from *Bifidobacterium longum* subsp. *longum* NCC2705 efficiently and selectively cleaves the N-glycan-derived disaccharide Man-β-1,4-GlcNAc (kcat/KM = 44.3 s-1 mM-1)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9025-43-8
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SMILES
[b-Mannosidase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[2]. Gytz H, et al. The structure of mammalian β-mannosidase provides insight into β-mannosidosis and nystagmus. FEBS J. 2019;286(7):1319-1331. [Content Brief]
[3]. Cooper A, Sardharwalla IB, Roberts MM. Human beta-mannosidase deficiency. N Engl J Med. 1986 Nov 6;315(19):1231. [Content Brief]
[4]. Cordeiro RL, et al. N-glycan Utilization by Bifidobacterium Gut Symbionts Involves a Specialist β-Mannosidase. J Mol Biol. 2019 Feb 15;431(4):732-747. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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