2. Others
  3. Fluorescent Dye
  4. 1,8-Diazafluoren-9-one

1,8-Diazafluoren-9-one  (Synonyms: DFO)

Cat. No.: HY-D0903 Purity: 99.61%
COA
SDS
Handling Instructions Technical Support

1,8-Diazafluoren-9-one (DFO) is a chemical that is used to find fingerprints on porous surfaces. 1,8-Diazafluoren-9-one reacts with amino acids present in the fingerprint to form highly fluorescent derivatives (Ex/Em = ~470/570).

For research use only. We do not sell to patients.

1,8-Diazafluoren-9-one

1,8-Diazafluoren-9-one Chemical Structure

CAS No. : 54078-29-4

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 mg In-stock
50 mg In-stock
100 mg In-stock
250 mg In-stock
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

1,8-Diazafluoren-9-one Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

1,8-Diazafluoren-9-one (DFO) is a chemical that is used to find fingerprints on porous surfaces. 1,8-Diazafluoren-9-one reacts with amino acids present in the fingerprint to form highly fluorescent derivatives (Ex/Em = ~470/570).

In Vitro

1,2-indanedione (1,2-IND) and 1,8-diazafluoren-9-one (DFO) are used in the forensic field to enhance latent fingerprints deposited on porous surfaces due to the formation of fluorescent products by reacting with the amino acids present in the papillary exudate. DFO has been the most used because of its excellent enhancing properties, even if it is more expensive and relatively toxic in comparison with the 1,2-IND[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1,8-Diazafluoren-9-one Related Antibodies
Molecular Weight

182.18

Formula

C11H6N2O
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C2=NC=CC=C2C3=CC=CN=C31
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (137.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.4891 mL 27.4454 mL 54.8908 mL
5 mM 1.0978 mL 5.4891 mL 10.9782 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.61%

SDS (393 KB)

COA (257 KB) HNMR (244 KB) RP-HPLC (246 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.4891 mL 27.4454 mL 54.8908 mL 137.2269 mL
5 mM 1.0978 mL 5.4891 mL 10.9782 mL 27.4454 mL
10 mM 0.5489 mL 2.7445 mL 5.4891 mL 13.7227 mL
15 mM 0.3659 mL 1.8297 mL 3.6594 mL 9.1485 mL
20 mM 0.2745 mL 1.3723 mL 2.7445 mL 6.8613 mL
25 mM 0.2196 mL 1.0978 mL 2.1956 mL 5.4891 mL
30 mM 0.1830 mL 0.9148 mL 1.8297 mL 4.5742 mL
40 mM 0.1372 mL 0.6861 mL 1.3723 mL 3.4307 mL
50 mM 0.1098 mL 0.5489 mL 1.0978 mL 2.7445 mL
60 mM 0.0915 mL 0.4574 mL 0.9148 mL 2.2871 mL
80 mM 0.0686 mL 0.3431 mL 0.6861 mL 1.7153 mL
100 mM 0.0549 mL 0.2745 mL 0.5489 mL 1.3723 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

1,8-Diazafluoren-9-one Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1,8-Diazafluoren-9-one54078-29-4DFOFluorescent DyeInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
1,8-Diazafluoren-9-one
Cat. No.:
HY-D0903
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.