2-Aminoethyl-mono-amide-DOTA-tris(tBu ester)
Based on 1 Customer Validation
2-Aminoethyl-mono-amide-DOTA-tris(tBu ester) is a metal chelator precursor containing a DOTA macrocyclic structure. DOTA can form highly stable complexes with metal ions (such as 68Ga, 177Lu) through four nitrogen atoms and four carboxylic acid groups to mediate targeted delivery of radionuclides. The tert-butyl ester group (tBu ester) of 2-Aminoethyl-mono-amide-DOTA-tris(tBu ester) also protects the carboxylic acid group during synthesis, and forms a free carboxyl group after deprotection reaction for coupling with targeting molecules (such as antibodies, peptides). 2-Aminoethyl-mono-amide-DOTA-tris(tBu ester) may be combined with tumor pre-targeting systems through bioorthogonal reactions (such as reverse electron demand Diels-Alder reaction) to study radioactive imaging or therapy of tumor tissues, and is mainly used in tumor pre-targeting research in the field of nuclear medicine.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 173308-19-5
- Formula: C30H58N6O7
- Molecular Weight:614.82
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-128890
DOTA-NHS-ester -
HY-W034551
DOTA-tri(t-butyl ester) -
HY-100138R
2-Aminoethyl-mono-amide-DOTA-tris(tBu ester) (Standard) -
HY-12692
DO3A tert-Butyl ester -
HY-W053583
Tetraxetan -
HY-W007656
Cyclen -
HY-W087027
Maleimide-NOTA -
HY-W614269
DOTA-GA(tBu)4 -
HY-W250574
NOTA-bis(tBu)ester -
HY-133540
Maleimide-DOTA -
HY-134797
p-SCN-Bn-deferoxamine -
HY-48876
p-NH2-Bn-DOTA
All Radionuclide-Drug Conjugates (RDCs) Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
RDC Bifunctional Chelator |
Cleavable Linker |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 173308-19-5
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Appearance Solid
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Molecular Weight 614.82
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Formula C30H58N6O7
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Color White to off-white
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SMILES
O=C(OC(C)(C)C)CN1CCN(CC(OC(C)(C)C)=O)CCN(CC(NCCN)=O)CCN(CC(OC(C)(C)C)=O)CC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (162.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6265 mL | 8.1325 mL | 16.2649 mL | 40.6623 mL |
| 5 mM | 0.3253 mL | 1.6265 mL | 3.2530 mL | 8.1325 mL | |
| 10 mM | 0.1626 mL | 0.8132 mL | 1.6265 mL | 4.0662 mL | |
| 15 mM | 0.1084 mL | 0.5422 mL | 1.0843 mL | 2.7108 mL | |
| 20 mM | 0.0813 mL | 0.4066 mL | 0.8132 mL | 2.0331 mL | |
| 25 mM | 0.0651 mL | 0.3253 mL | 0.6506 mL | 1.6265 mL | |
| 30 mM | 0.0542 mL | 0.2711 mL | 0.5422 mL | 1.3554 mL | |
| 40 mM | 0.0407 mL | 0.2033 mL | 0.4066 mL | 1.0166 mL | |
| 50 mM | 0.0325 mL | 0.1626 mL | 0.3253 mL | 0.8132 mL | |
| 60 mM | 0.0271 mL | 0.1355 mL | 0.2711 mL | 0.6777 mL | |
| 80 mM | 0.0203 mL | 0.1017 mL | 0.2033 mL | 0.5083 mL | |
| 100 mM | 0.0163 mL | 0.0813 mL | 0.1626 mL | 0.4066 mL |
2-Aminoethyl-mono-amide-DOTA-tris(tBu ester) Related Classifications
HY-100138 Related Classifications
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