2-Methoxypyrazine
Based on 1 Customer Validation
2-Methoxypyrazine is an active compound. 2-Methoxypyrazine can be used for the research of various biochemical studies.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 3149-28-8
- Formula: C5H6N2O
- Molecular Weight:110.12
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
Chemical Information
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CAS No. 3149-28-8
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Appearance Liquid
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Molecular Weight 110.12
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Formula C5H6N2O
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Color Colorless to light yellow
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SMILES
COC1=CN=CC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (908.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (767 KB)
- English - EN (767 KB)
- Français - FR (767 KB)
- Deutsch - DE (767 KB)
- Norwegian - NO (767 KB)
- Español - ES (767 KB)
- Swedish - SV (767 KB)
- Italian - IT (767 KB)
- Korean - KR (767 KB)
- Portuguese - PT (767 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 9.0813 mL | 45.4067 mL | 90.8133 mL | 227.0333 mL |
| 5 mM | 1.8163 mL | 9.0813 mL | 18.1627 mL | 45.4067 mL | |
| 10 mM | 0.9081 mL | 4.5407 mL | 9.0813 mL | 22.7033 mL | |
| 15 mM | 0.6054 mL | 3.0271 mL | 6.0542 mL | 15.1356 mL | |
| 20 mM | 0.4541 mL | 2.2703 mL | 4.5407 mL | 11.3517 mL | |
| 25 mM | 0.3633 mL | 1.8163 mL | 3.6325 mL | 9.0813 mL | |
| 30 mM | 0.3027 mL | 1.5136 mL | 3.0271 mL | 7.5678 mL | |
| 40 mM | 0.2270 mL | 1.1352 mL | 2.2703 mL | 5.6758 mL | |
| 50 mM | 0.1816 mL | 0.9081 mL | 1.8163 mL | 4.5407 mL | |
| 60 mM | 0.1514 mL | 0.7568 mL | 1.5136 mL | 3.7839 mL | |
| 80 mM | 0.1135 mL | 0.5676 mL | 1.1352 mL | 2.8379 mL | |
| 100 mM | 0.0908 mL | 0.4541 mL | 0.9081 mL | 2.2703 mL |