3-Arm PEG40000-Azide
3-Arm PEG40000-Azide (3-Arm PEG40000-N3) is a three-armed PEG derivative. The core structure of 3-Arm PEG40000-Azide consists of three PEG chains, each terminal of which is modified with an azide (-N3) group. The azide group in 3-Arm PEG40000-Azide can undergo click chemistry reactions and is used for applications such as constructing targeted drug delivery systems and nanoparticle modification.
For research use only. We do not sell to patients.
- Molecular Weight:40000 (Average)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
In Vitro
The structure of PEG-Azide contains two key parts that work synergistically:
Azide group: can undergo a copper-catalyzed azido-yne cycloaddition reaction (CuAAc) with molecules containing an Alkyne group. It can also undergo ring-strain-driven acetylene-azidocycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
PEG chain: As a hydrophilic spacer, it forms a hydration layer on the particle surface, reducing non-specific adsorption and immune system recognition and clearance, thus prolonging the carrier's circulation time in vivo.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 40000 (Average)
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SMILES
CCC(COCCOCCN=[N+]=[N-])(COCCOCCN=[N+]=[N-])COCCOCCN=[N+]=[N-].[n].[n].[n]
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Synonyms
3-Arm PEG40000-N3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3-Arm PEG40000-Azide Related Classifications
HY-W1049013D Related Classifications
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