4-(Bromomethyl)benzoic acid
Based on 1 Customer Validation
4-(Bromomethyl)benzoic acid (4-Carboxybenzyl bromide) is an antiparasitic agent with low activity against Leishmania amazonensis and Trypanosoma cruzi. 4-(Bromomethyl)benzoic acid has certain cytotoxicity to PBMCs, with an IC50 of 88.8 μM.
For research use only. We do not sell to patients.
- Purity: 99.14%
- CAS No.: 6232-88-8
- Formula: C8H7BrO2
- Molecular Weight:215.04
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Parasite Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| L929 | IC50 |
500 μM
Compound: 1
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Trypanocidal activity against intracellular trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis
Trypanocidal activity against intracellular trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis
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[PMID: 21940071] |
| L929 | IC50 |
>1000 μM
Compound: 1
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Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis
Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis
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[PMID: 21940071] |
| PBMC | IC50 |
88.8 μM
Compound: 1
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Inhibition of cell proliferation of phytohemagglutinin-induced human PBMC
Inhibition of cell proliferation of phytohemagglutinin-induced human PBMC
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[PMID: 21940071] |
Chemical Information
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CAS No. 6232-88-8
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Appearance Solid
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Molecular Weight 215.04
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Formula C8H7BrO2
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Color White to off-white
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SMILES
BrCC1=CC=C(C(O)=O)C=C1
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Synonyms
4-Carboxybenzyl bromide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (465.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (23.25 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.6503 mL | 23.2515 mL | 46.5030 mL | 116.2574 mL |
| 5 mM | 0.9301 mL | 4.6503 mL | 9.3006 mL | 23.2515 mL | |
| 10 mM | 0.4650 mL | 2.3251 mL | 4.6503 mL | 11.6257 mL | |
| 15 mM | 0.3100 mL | 1.5501 mL | 3.1002 mL | 7.7505 mL | |
| 20 mM | 0.2325 mL | 1.1626 mL | 2.3251 mL | 5.8129 mL | |
| 25 mM | 0.1860 mL | 0.9301 mL | 1.8601 mL | 4.6503 mL | |
| 30 mM | 0.1550 mL | 0.7750 mL | 1.5501 mL | 3.8752 mL | |
| 40 mM | 0.1163 mL | 0.5813 mL | 1.1626 mL | 2.9064 mL | |
| 50 mM | 0.0930 mL | 0.4650 mL | 0.9301 mL | 2.3251 mL | |
| 60 mM | 0.0775 mL | 0.3875 mL | 0.7750 mL | 1.9376 mL | |
| 80 mM | 0.0581 mL | 0.2906 mL | 0.5813 mL | 1.4532 mL | |
| 100 mM | 0.0465 mL | 0.2325 mL | 0.4650 mL | 1.1626 mL |
4-(Bromomethyl)benzoic acid Related Classifications
HY-21496 Related Classifications
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