2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  3. 5-HT Receptor Tryptophan Hydroxylase
  4. 4-Chloro-L-phenylalanine

4-Chloro-L-phenylalanine  (Synonyms: L-PCPA)

Cat. No.: HY-W019599 Purity: 98.19%
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Handling Instructions Technical Support

4-Chloro-L-phenylalanine (L-PCPA) is a tryptophan hydroxylase inhibitor targeting TPH1 and TPH2, with the activity of blocking serotonin biosynthesis. 4-Chloro-L-phenylalanine reduces the levels of serotonin and its metabolites in the brain without impairing the survival of serotonergic neurons. 4-Chloro-L-phenylalanine enhances anhedonic, depression-like and anxiety-like behaviors in mice with depleted noradrenergic and dopaminergic neurons. 4-Chloro-L-phenylalanine acts as a decarboxylation substrate for aromatic L-amino acid decarboxylase from Bacillus atrophaeus. 4-Chloro-L-phenylalanine can be used in studies related to Parkinson's disease.

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4-Chloro-L-phenylalanine

4-Chloro-L-phenylalanine Chemical Structure

CAS No. : 14173-39-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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1 g In-stock
5 g In-stock
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Based on 1 publication(s) in Google Scholar

4-Chloro-L-phenylalanine Related Antibodies

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Description

4-Chloro-L-phenylalanine (L-PCPA) is a tryptophan hydroxylase inhibitor targeting TPH1 and TPH2, with the activity of blocking serotonin biosynthesis. 4-Chloro-L-phenylalanine reduces the levels of serotonin and its metabolites in the brain without impairing the survival of serotonergic neurons. 4-Chloro-L-phenylalanine enhances anhedonic, depression-like and anxiety-like behaviors in mice with depleted noradrenergic and dopaminergic neurons. 4-Chloro-L-phenylalanine acts as a decarboxylation substrate for aromatic L-amino acid decarboxylase from Bacillus atrophaeus. 4-Chloro-L-phenylalanine can be used in studies related to Parkinson's disease[1][2][3].

In Vitro

4-Chloro-L-phenylalanine (5 mM) is catalyzed for decarboxylation by purified AADC-BA from Bacillus atrophaeus[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Chloro-L-phenylalanine Related Antibodies
In Vivo

4-Chloro-L-phenylalanine (300 mg/kg; i.p.; once daily; 3 consecutive days) reduces central 5-HT and 5-HIAA levels in mice, decreases 5-HT metabolic rate, and induces despair-associated depressive-like behavior without altering serotonergic neuron counts, locomotor activity, anxiety, or spatial cognition[1].
4-Chloro-L-phenylalanine (150 mg/kg/d; i.p.; daily; 3 consecutive days) reverses enhanced serotonin signaling-induced intestinal mucosal growth and enteric neuroplasticity in SERTKO and WT-SSRI mice, while only reducing villus height without affecting other parameters in WT mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (male, 8 weeks of age)[1]
Dosage: 300 mg/kg
Administration: i.p.; once daily; 3 consecutive days
Result: Reduced frontal cortex 5-HT levels by 53% and 5-HIAA levels by 73% compared to saline controls; decreased the 5-HIAA/5-HT ratio by 42%.
Reduced hippocampus 5-HT levels by 57% and 5-HIAA levels by 76% compared to saline controls; decreased the 5-HIAA/5-HT ratio by 46%.
Did not significantly change the number of serotonin transporter-immunoreactive (SERT-ir) neurons in the dorsal raphe nucleus compared to saline controls.
Significantly increased immobility time in both the forced swim test and tail suspension test.
Did not alter locomotor activity, sucrose preference, anxiety-related behavior, or spatial learning/memory compared to saline controls.
Molecular Weight

199.64

Formula

C9H10ClNO2
CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 10.29 mg/mL (51.54 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.0090 mL 25.0451 mL 50.0902 mL
5 mM 1.0018 mL 5.0090 mL 10.0180 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.19%

SDS (480 KB)

COA (246 KB) HNMR (244 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.0090 mL 25.0451 mL 50.0902 mL 125.2254 mL
5 mM 1.0018 mL 5.0090 mL 10.0180 mL 25.0451 mL
10 mM 0.5009 mL 2.5045 mL 5.0090 mL 12.5225 mL
15 mM 0.3339 mL 1.6697 mL 3.3393 mL 8.3484 mL
20 mM 0.2505 mL 1.2523 mL 2.5045 mL 6.2613 mL
25 mM 0.2004 mL 1.0018 mL 2.0036 mL 5.0090 mL
30 mM 0.1670 mL 0.8348 mL 1.6697 mL 4.1742 mL
40 mM 0.1252 mL 0.6261 mL 1.2523 mL 3.1306 mL
50 mM 0.1002 mL 0.5009 mL 1.0018 mL 2.5045 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Chloro-L-phenylalanine Related Classifications

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Keywords:

4-Chloro-L-phenylalanine14173-39-8L-PCPA5-HT ReceptorTryptophan HydroxylaseSerotonin Receptor5-hydroxytryptamine Receptoraromatic L-amino acid decarboxylasenoradrenergic depletiontryptophan hydroxylaseBacillus atrophaeusmiceserotonin biosynthesisTPH1dopaminergic depletionserotonergic neuronTPH2Inhibitorinhibitorinhibit

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