PCPA methyl ester hydrochloride
Based on 5 publication(s) in Google Scholar
PCPA methyl ester hydrochloride (4-Chloro-DL-phenylalanine methyl ester hydrochloride), a reversible tryptophan hydroxylase inhibitor, is a serotonin (5-HT) synthesis inhibitor. PCPA methyl ester hydrochloride crosses the blood brain barrier and reduces 5-HT central availability.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 14173-40-1
- Formula: C10H13Cl2NO2
- Molecular Weight:250.12
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) PCPA methyl ester hydrochloride
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Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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IHC
All 5-HT Receptor Isoforms
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Biological Activity
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serotonin |
Tryptophan Hydroxylase |
In C57BL/6 elite male mice, 5-HT levels decreased by 85% and 55% in the hippocampus of mice treated with oral or ip PCPA methyl ester hydrochloride, respectively, whereas in the prefrontal cortex, 5-HT levels decreased by 65% (oral) and 50% (ip)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 elite male mice (3 months old)[3]
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Dosage:500 mg/kg per day for the first 2 days and 250 mg/kg for the rest of the treatment
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Administration:Oral administration; daily; for 8 weeks
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Result:Serotonin levels were significantly decreased in both hippocampus and cortex.
Chemical Information
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CAS No. 14173-40-1
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Appearance Solid
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Molecular Weight 250.12
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Formula C10H13Cl2NO2
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Color White to off-white
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SMILES
NC(CC1=CC=C(Cl)C=C1)C(OC)=O.Cl
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Synonyms
4-Chloro-DL-phenylalanine methyl ester hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Res
Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons. [Abstract]2022 Jun;32(6):555-569. PMID: 35379903
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2022 Jun;32(6):555-569. [Abstract]
The tryptophan hydroxylase inhibitor PCPA methyl ester hydrochloride (pCPA) most significantly decreased the ratios of ISCs in small intestines (SI).
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2022 Jun;32(6):555-569. [Abstract]
The tryptophan hydroxylase inhibitor PCPA methyl ester hydrochloride (pCPA) most significantly decreased the ratios of ISCs in colons.
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2022 Jun;32(6):555-569. [Abstract]
PCPA methyl ester hydrochloride (pCPA)-treated mice were treated with 10 Gy’s radiation, and sacrificed at indicated time points. Intestinal tissues were collected for H&E staining (upper panel). Numbers of intact crypts were shown in lower panel (means ± SD). Five 2-month-old mice were used and 20 fields were taken for each group. 150 mg/kg pCPA was intraperitoneally injected.
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2022 Jun;32(6):555-569. [Abstract]
Two-month old mice were treated with 5-HT inhibitor PCPA methyl ester hydrochloride (pCPA) for 36 h and 72 h, and representative H&E images of SI and colon were shown in left panel. 100 fields from five mice were observed and shown in right panel. 150 mg/kg PCPA methyl ester hydrochloride (pCPA) was intraperitoneally injected.
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J Transl Med
Central 5-HTergic hyperactivity induces myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)-like pathophysiology. [Abstract]2024 Jan 8;22(1):34. PMID: 38191373 -
Neurochem Res
Banxia Shumi Decoction Improves Sleep and Neural Function in Insomnia Male Rats via BDNF/TrkB/CREB-Dependent Melatonin Signaling Activation. [Abstract]2025 Sep 24;50(5):306. PMID: 40991085
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 24;50(5):306. [Abstract]
To explore the effects of BSXMD treatment on insomnia male rats, PCPA methyl ester hydrochloride (PCPA)-induced insomnia modeling, treatment intervention and behavioral tests were performed according to the timeline shown in Fig.
PCPA methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 24;50(5):306. [Abstract]
Compared with normal male rats, rats in the insomnia model group (PCPA methyl ester hydrochloride, 350 mg/kg, ip) exhibited dull fur and a significant decrease in body weight. Following treatment with BSXMD or diazepam, the fur of the insomniac rats became smooth and lustrous, and their body weight increased.
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Neurosci Lett
Ferulic acid methylester improves the comorbidity of insomnia and anxiety in a rat model of PCPA-induced sleep disorder by activating DRN 5-HT neurons. [Abstract]2025 May 27:138276. PMID: 40441597 -
Solvent & Solubility
DMSO : 50 mg/mL (199.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (199.90 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (19.99 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (19.99 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (399.80 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Dool-Ri Oh, et al. Sedative and hypnotic effects of Vaccinium bracteatum Thunb. through the regulation of serotonegic and GABA A-ergic systems: Involvement of 5-HT 1A receptor agonistic activity. Biomed Pharmacother. 2019 Jan;109:2218-2227. [Content Brief]
[2]. Fabio Bellia, et al. Transient serotonin depletion at adolescence, but not at early infancy, reduced subsequent anxiety-like behavior and alcohol intake in female mice. Psychopharmacology (Berl). 2021 Jan;238(1):215-225. [Content Brief]
[3]. ocío B Foltran, et al. Neurochemical, Behavioral, and Neurogenic Validation of a Hyposerotonergic Animal Model by Voluntary Oral Consumption of para-Chlorophenylalanine. ACS Chem Neurosci. 2020 Mar 18;11(6):952-959. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 3.9980 mL | 19.9900 mL | 39.9800 mL | 99.9500 mL |
| 5 mM | 0.7996 mL | 3.9980 mL | 7.9960 mL | 19.9900 mL | |
| 10 mM | 0.3998 mL | 1.9990 mL | 3.9980 mL | 9.9950 mL | |
| 15 mM | 0.2665 mL | 1.3327 mL | 2.6653 mL | 6.6633 mL | |
| 20 mM | 0.1999 mL | 0.9995 mL | 1.9990 mL | 4.9975 mL | |
| 25 mM | 0.1599 mL | 0.7996 mL | 1.5992 mL | 3.9980 mL | |
| 30 mM | 0.1333 mL | 0.6663 mL | 1.3327 mL | 3.3317 mL | |
| 40 mM | 0.1000 mL | 0.4998 mL | 0.9995 mL | 2.4988 mL | |
| 50 mM | 0.0800 mL | 0.3998 mL | 0.7996 mL | 1.9990 mL | |
| 60 mM | 0.0666 mL | 0.3332 mL | 0.6663 mL | 1.6658 mL | |
| 80 mM | 0.0500 mL | 0.2499 mL | 0.4998 mL | 1.2494 mL | |
| 100 mM | 0.0400 mL | 0.1999 mL | 0.3998 mL | 0.9995 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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