4-Methylumbelliferyl oleate

Name: 4-Methylumbelliferyl oleate
Brand: MedChemExpress
SKU: HY-117095
Rating: 4.5 (0 reviews)

Cat. No.: HY-117095 Pureza: 99.58%: Ficha de datos
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4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).

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4-Methylumbelliferyl oleate Estructura química

No. CAS : 18323-58-5

Tamaño	Stock	Cantidad
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid or liquid
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Descripciòn

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm)^[1]^[2].

In Vitro

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Prepare the reaction system: Add the following components in sequence to the microplate or reaction tube:
50 μL 0.1 mM 4-MU oleate (substrate)
20 μL McIlvane buffer (pH 7.4)
5 μL sample solution to be tested (dissolved in an appropriate solvent)
2. Add the enzyme solution: Add 25 μL porcine pancreatic lipase to initiate the reaction.
3. Volume Adjustment: If necessary, add buffer solution or deionized water to replenish the total volume of the reaction to 100 μL.
4. Incubation: Place the reaction system at 37°C for 10 minutes.
5. Detection: Measure the fluorescence intensity of the released 4-methylumbelliferone (4-MU).
Excitation wavelength: 320 nm Emission wavelength: 450 nm

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

440.61

Fòrmula

C₂₈H₄₀O₄

No. CAS

18323-58-5

Appearance

Solid-Liquid Mixture

Color

White to off-white

SMILES

O=C1C=C(C2=C(O1)C=C(C=C2)OC(CCCCCCC/C=C\CCCCCCCC)=O)C

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (226.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2696 mL	11.3479 mL	22.6958 mL
5 mM	0.4539 mL	2.2696 mL	4.5392 mL
10 mM	0.2270 mL	1.1348 mL	2.2696 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.58%

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Ficha de datos (267 KB) SDS (393 KB)

COA (239 KB) HNMR (240 KB) RP-HPLC (244 KB) LCMS (93 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Zhang J, et al. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo. Nutr Res Pract. 2008 Winter;2(4):200-3. [Content Brief]

[2]. Koster JF, et al. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver. Biochim Biophys Acta. 1980 Apr 18;618(1):98-105. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2696 mL	11.3479 mL	22.6958 mL	56.7395 mL
	5 mM	0.4539 mL	2.2696 mL	4.5392 mL	11.3479 mL
	10 mM	0.2270 mL	1.1348 mL	2.2696 mL	5.6740 mL
	15 mM	0.1513 mL	0.7565 mL	1.5131 mL	3.7826 mL
	20 mM	0.1135 mL	0.5674 mL	1.1348 mL	2.8370 mL
	25 mM	0.0908 mL	0.4539 mL	0.9078 mL	2.2696 mL
	30 mM	0.0757 mL	0.3783 mL	0.7565 mL	1.8913 mL
	40 mM	0.0567 mL	0.2837 mL	0.5674 mL	1.4185 mL
	50 mM	0.0454 mL	0.2270 mL	0.4539 mL	1.1348 mL
	60 mM	0.0378 mL	0.1891 mL	0.3783 mL	0.9457 mL
	80 mM	0.0284 mL	0.1418 mL	0.2837 mL	0.7092 mL
	100 mM	0.0227 mL	0.1135 mL	0.2270 mL	0.5674 mL