4-Nitrophenyl β-D-mannopyranoside  (Synonyms: p-Nitrophenyl β-D-Mannopyranoside)

4-Nitrophenyl β-D-mannopyranoside is a useful substrate for β-D-mannopyranosidase. 4-Nitrophenyl β-D-mannopyranoside is also a substrate for GH1-glucosidase (EaBgl1A) and α-L-rhamnosidase^[1][2][3].

301.25

C₁₂H₁₅NO₈

35599-02-1

Solid

White to off-white

OC[C@](O1)([H])[C@](O)([H])[C@@](O)([H])[C@@](O)([H])[C@]1([H])OC2=CC=C([N+]([O-])=O)C=C2

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Rosenfield L, et al. A practical preparation of p-nitrophenyl β-d-mannopyranoside. Carbohydrate Research, 1976, 46(1): 155-158.

  [2]. Crespim E, et al. A novel cold-adapted and glucose-tolerant GH1 β-glucosidase from Exiguobacterium antarcticum B7. Int J Biol Macromol. 2016 Jan;82:375-80.  [Content Brief]

  [3]. Maruvada R, et al. Evaluation of enzymatic and non-enzymatic softening in low salt cucumber fermentations. International Journal of Food Science and Technology, 2009, 44(6): 1108-1117.