4-Pentynoyl-Coenzyme A TFA  (Synonyms: 4-Pentynoyl-CoA TFA)

4-Pentynoyl-Coenzyme A (4-Pentynoyl-CoA) TFA is an analog of the natural substrate acetyl-CoA (Acetyl-CoA) (HY-114293). 4-Pentynoyl-Coenzyme A TFA can replace acetyl-CoA to be recognized and utilized by lysine acetyltransferases such as p300. 4-Pentynoyl-Coenzyme A TFA can be conjugated to azide probes carrying fluorescent groups (e.g., Rhodamine-azide) or biotin (Biotin-azide) via Cu (I)-catalyzed azide-alkyne cycloaddition (CuAAC)^[1].

4-Pentynoyl-Coenzyme A TFA can transfer its 4-pentynoyl group to the lysine residues of p300 protein substrates under the catalysis of p300, thereby enabling fluorescent labeling and identification via CuI-catalyzed alkyne-azide cycloaddition reaction^[1].
4-Pentynoyl-Coenzyme A TFA enables fluorescent analysis and identification of p300 protein substrates and their lysine acetylation sites in in vitro cell extracts via bioorthogonal proteomic approaches^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

961.64

C₂₈H₄₁F₃N₇O₁₉P₃S

OC(C(F)(F)F)=O.NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](OP(O)(O)=O)[C@@H](COP(OP(OCC(C)([C@H](C(NCCC(NCCSC(CCC#C)=O)=O)=O)O)C)(O)=O)(O)=O)O3

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• [1]. Yang YY, et al. Identification of lysine acetyltransferase p300 substrates using 4-pentynoyl-coenzyme A and bioorthogonal proteomics. Bioorg Med Chem Lett. 2011;21(17):4976-4979.