Acetyl coenzyme A
Based on 7 publication(s) in Google Scholar
Acetyl-coenzyme A (Acetyl-CoA) is a membrane-impermeant central metabolic intermediate, participates in the TCA cycle and oxidative phosphorylation metabolism. Acetyl-coenzyme A, regulates various cellular mechanisms by providing (sole donor) acetyl groups to target amino acid residues for post-translational acetylation reactions of proteins. Acetyl Coenzyme A is also a key precursor of lipid synthesis.
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- CAS No.: 72-89-9
- Formule: C23H38N7O17P3S
- Masse moléculaire:809.57
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Acetyl coenzyme A
More- Cancer Res. 2025 Sep 3. [Abstract]
- Bone Res. 2024 Aug 28;12(1):49. [Abstract]
- Sci Adv. 2026 May 15;12(20):eadz3835. [Abstract]
- Cell Rep. 2025 Jan 10;44(1):115203. [Abstract]
- Chin Med. 2026 Jun 8;21(1):162. [Abstract]
- J Cell Physiol. 2023 Mar;238(3):610-630. [Abstract]
- bioRxiv. 2026 May 20:2026.05.19.726225. [Abstract]
Voir tous les produits spécifiques à Isoform Endogenous Metabolite
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Activité biologique
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Acetyl coenzyme A increases cytoplasmic protein acetylation in starved U2OS cells while reducing starvation-induced autophagic fluxes. (U2OS cells stably expressing GFP-LC3 and are microinjected with Acetyl coenzyme A; incubated in nutrient-free conditions in the presence of 100 nM BafA1 and fixed after 3 h)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 72-89-9
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Masse moléculaire 809.57
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Formule C23H38N7O17P3S
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SMILES
CC(SCCNC(CCNC([C@H](O)C(C)(C)COP(OP(O)(OC[C@H]1O[C@@H](N(C=N2)C3=C2C(N)=NC=N3)[C@H](O)[C@@H]1OP(O)(O)=O)=O)(O)=O)=O)=O)=O
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Synonyms
Acetyl-CoA
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (7)
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Journal Impact Factor
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Most Recent
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Cancer Res
A Citrate Synthase Splice Variant Rewires the TCA Cycle to Promote Colorectal Cancer Progression. [Abstract]2025 Sep 3. PMID: 40900036 -
Bone Res
2024 Aug 28;12(1):49. PMID: 39198395 -
Sci Adv
mTOR-NAA10-C7orf50 axis senses nutritional status to coordinate ribosome biogenesis and autophagy. [Abstract]2026 May 15;12(20):eadz3835. PMID: 42139339 -
Cell Rep
Ergosterol alleviates hepatic steatosis and insulin resistance via promoting fatty acid β-oxidation by activating mitochondrial ACSL1. [Abstract]2025 Jan 10;44(1):115203. PMID: 39799570 -
Chin Med
Chuangling Ye mitigates diabetic foot ulcer through suppressing keratinocyte ferroptosis via inhibiting ACSS2/ACSL4 axis. [Abstract]2026 Jun 8;21(1):162. PMID: 42260608 -
J Cell Physiol
Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics. [Abstract]2023 Mar;238(3):610-630. PMID: 36745473 -
bioRxiv
A liquid chromatography-mass spectrometry method to quantify total Coenzyme A concentration and isotopic labeling. [Abstract]2026 May 20:2026.05.19.726225. PMID: 42239474
Protocole
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
Pureté et documentation
Références
[1]. Choudhary C, et al. The growing landscape of lysine acetylation links metabolism and cell signalling. Nat Rev Mol Cell Biol. 2014 Aug;15(8):536-50. [Content Brief]
[2]. Mariño G, et al. Regulation of autophagy by cytosolic acetyl-coenzyme A. Mol Cell. 2014 Mar 6;53(5):710-25. [Content Brief]
[3]. Zhu H, et al. Cardiac autophagy is a maladaptive response to hemodynamic stress. J Clin Invest. 2007 Jul;117(7):1782-93. [Content Brief]
[4]. Pietrocola F, et al. Acetyl coenzyme A: a central metabolite and second messenger. Cell Metab. 2015 Jun 2;21(6):805-21. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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