5,6-trans-Vitamin D3
Based on 1 Customer Validation
5,6-trans-Vitamin D3 (5,6-trans-Cholecalciferol;5,6-trans-Colecalciferol) is a photoproduct of vitamin D3. Vitamin D3 is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.
For research use only. We do not sell to patients.
- Purity : 99.81%
- CAS No.: 22350-41-0
- Formula: C27H44O
- Molecular Weight:384.64
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-76542
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CalcipotriolVitamin D3 Analog -
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DoxercalciferolVD/VDR Activator -
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Retinyl acetateAnticancer Agent -
HY-32339
MaxacalcitolVDR Agonist -
HY-10001A
Calcipotriol monohydrateVitamin D3 Analog -
HY-15398AR
5,6-trans-Vitamin D3 (Standard)Photoproduct of Vitamin D3 -
HY-32350
ErcalcitriolVDR Agonist
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-29 | GI50 |
>100 μM
Compound: 2, trans-VD3
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Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay
Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay
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[PMID: 22687748] |
| U-87MG ATCC | GI50 |
46 μM
Compound: 2, trans-VD3
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Growth inhibition of human U87MG cells after 72 hrs by MTS assay
Growth inhibition of human U87MG cells after 72 hrs by MTS assay
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[PMID: 22687748] |
In Vitro
Normal human keratinocytes in the cultured human keratinocytes by determining their influence on 3H-thymidine incorporation into DNA.5,6-trans-vitamin D3 only induces significant inhibition at 10(-6) M. The dissociation constants of vitamin D receptor (VDR) for 5,6-trans-vitamin D3 is 560 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 22350-41-0
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Appearance Solid
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Molecular Weight 384.64
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Formula C27H44O
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Color White to light yellow
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SMILES
O[C@@H]1C/C(C(CC1)=C)=C\C=C2[C@]3([H])CC[C@H]([C@H](C)CCCC(C)C)[C@@]3(C)CCC\2
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Synonyms
5,6-trans-Cholecalciferol; 5,6-trans-Colecalciferol; Cholecalciferol EP Impurity A
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
Ethanol : 16.67 mg/mL (43.34 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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iPSC cell differentiation
Induced pluripotent stem cells (iPSCs) are a type of cell that has similar properties to embryonic stem cells through somatic cell reprogramming.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.5998 mL | 12.9992 mL | 25.9983 mL | 64.9958 mL |
| 5 mM | 0.5200 mL | 2.5998 mL | 5.1997 mL | 12.9992 mL | |
| 10 mM | 0.2600 mL | 1.2999 mL | 2.5998 mL | 6.4996 mL | |
| 15 mM | 0.1733 mL | 0.8666 mL | 1.7332 mL | 4.3331 mL | |
| 20 mM | 0.1300 mL | 0.6500 mL | 1.2999 mL | 3.2498 mL | |
| 25 mM | 0.1040 mL | 0.5200 mL | 1.0399 mL | 2.5998 mL | |
| 30 mM | 0.0867 mL | 0.4333 mL | 0.8666 mL | 2.1665 mL | |
| 40 mM | 0.0650 mL | 0.3250 mL | 0.6500 mL | 1.6249 mL |
5,6-trans-Vitamin D3 Related Classifications
HY-15398A Related Classifications
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