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  3. 7-Amino-4-methylcoumarin-3-acetic acid

7-Amino-4-methylcoumarin-3-acetic acid 

Cat. No.: HY-D0025 Purity: 99.89%
COA Handling Instructions

7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm).

For research use only. We do not sell to patients.

7-Amino-4-methylcoumarin-3-acetic acid Chemical Structure

7-Amino-4-methylcoumarin-3-acetic acid Chemical Structure

CAS No. : 106562-32-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 364 In-stock
Solution
10 mM * 1 mL in DMSO USD 364 In-stock
Solid
1 mg USD 142 In-stock
5 mg USD 300 In-stock
10 mg USD 450 In-stock
25 mg USD 950 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
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  • Purity & Documentation

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Description

7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm)[1].

Molecular Weight

233.22

Formula

C12H11NO4

CAS No.
Appearance

Solid

Color

Off-white to light brown

Emission (Em)

454

Excitation (Ex)

344

SMILES

O=C(O)CC1=C(C)C2=CC=C(N)C=C2OC1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (535.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2878 mL 21.4390 mL 42.8780 mL
5 mM 0.8576 mL 4.2878 mL 8.5756 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2878 mL 21.4390 mL 42.8780 mL 107.1949 mL
5 mM 0.8576 mL 4.2878 mL 8.5756 mL 21.4390 mL
10 mM 0.4288 mL 2.1439 mL 4.2878 mL 10.7195 mL
15 mM 0.2859 mL 1.4293 mL 2.8585 mL 7.1463 mL
20 mM 0.2144 mL 1.0719 mL 2.1439 mL 5.3597 mL
25 mM 0.1715 mL 0.8576 mL 1.7151 mL 4.2878 mL
30 mM 0.1429 mL 0.7146 mL 1.4293 mL 3.5732 mL
40 mM 0.1072 mL 0.5360 mL 1.0719 mL 2.6799 mL
50 mM 0.0858 mL 0.4288 mL 0.8576 mL 2.1439 mL
60 mM 0.0715 mL 0.3573 mL 0.7146 mL 1.7866 mL
80 mM 0.0536 mL 0.2680 mL 0.5360 mL 1.3399 mL
100 mM 0.0429 mL 0.2144 mL 0.4288 mL 1.0719 mL
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7-Amino-4-methylcoumarin-3-acetic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
7-Amino-4-methylcoumarin-3-acetic acid
Cat. No.:
HY-D0025
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