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Decursin  (Synonyms: (+)-Decursin)

Cat. No.: HY-18981 Purity: 99.99%
COA Handling Instructions

Decursin ((+)-Decursin) is a potent anti-tumor agent. Decursin also is a cytotoxic agent and a potent protein kinase C activator. Decursin induces apoptosis and cell cycle arrest at G1 phase. Decursin decreases the expression of CDK2, CDK4, CDK6, cyclin D1 protein at 48 h. Decursin inhibits cell proliferation and migration. Decursin shows anti-tumor, anti-inflammatory and analgesic activities.

For research use only. We do not sell to patients.

Decursin Chemical Structure

Decursin Chemical Structure

CAS No. : 5928-25-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
1 mg USD 47 In-stock
5 mg USD 100 In-stock
10 mg USD 150 In-stock
25 mg USD 330 In-stock
50 mg USD 590 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Decursin:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Decursin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Decursin ((+)-Decursin) is a potent anti-tumor agent. Decursin also is a cytotoxic agent and a potent protein kinase C activator. Decursin induces apoptosis and cell cycle arrest at G1 phase. Decursin decreases the expression of CDK2, CDK4, CDK6, cyclin D1 protein at 48 h. Decursin inhibits cell proliferation and migration. Decursin shows anti-tumor, anti-inflammatory and analgesic activities[1][2][3][4].

In Vitro

Decursin (0, 25, 50, 100 μM; 24, 28, 72, 96 h) inhibits cell growth in a dose- and time- dependent manner in DU145 cells[1].
Decursin (0, 25, 50, 100 μM; 24, 28, 72, 96 h) induces apoptosis and cell cycle arrest at G1 phase in DU145 cells, G1, S as well as G2-M arrests in PC-3 cells[1].
Decursin (0, 25, 50, 100 μM; 24, 48 h) decreases the expression of CDK2, CDK4, CDK6, cyclin D1 protein at 48 h in DU145 cells[1].
Decursin (0, 5, 20, 100 μM; 7 days) inhibits the proliferation and differentiation ability of AC133+ cells[2].
Decursin (0, 5, 20, 100 μM) inhibits SDF-1a-induced activation of Akt, ERK1/2, and eNOS in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: DU145 cells
Concentration: 0, 25, 50, 100 µM
Incubation Time: 24, 28, 72, 96 h
Result: Showed a dose- and time- dependent inhibition cell growth with 22% to 51%, 21%to 68%, 9% to 72%, 42% to 90% growth inhibition after 24, 48, 72, and 96 hours oftreatment, respectively. And caused 15%-45% cell death.

Cell Cycle Analysis[1]

Cell Line: DU145 cells
Concentration: 0, 25, 50, 100 µM
Incubation Time: 12-96 h
Result: Caused 52%, 65%, and 78% DU145 cells in G1phase.

Western Blot Analysis[1]

Cell Line: DU145 cells
Concentration: 0, 25, 50, 100 µM
Incubation Time: 24, 48 h
Result: Did not show any any alteration inprotein levels of CDK2, CDK4, CDK6, cyclin D1, and cyclin E, but dose-dependent decreased in the expression of these proteins except cyclin E at 48 h.
In Vivo

Decursin (4 mg/kg; s.c.; daily for 4 weeks) shows anti-tumor activity in mouse[2].
Decursin (50 mg/kg; intrathecal injection; three times at 2-day intervals, for 6 days) shows analgesic ability in paclitaxel-induced peripheral neuropathy in mouse[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice (LLC cells)[2]
Dosage: 4 mg/kg
Administration: S.c.; daily for 4 weeks
Result: Delayed tumor formation and dramatically decreased tumor growth by inhibition of angiogenesis through VEGFR-2 signaling pathway.
Animal Model: Eight-week-old adult C57BL/6J male and female mice[3]
Dosage: 50 mg/kg
Administration: Intrathecal injection; three times at 2-day intervals, for 6 days
Result: Demonstrated the analgesic ability in the in vivo model of paclitaxel-induced peripheral neuropathy.
Molecular Weight

328.36

Formula

C19H20O5

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C/C(C)=C\C(O[C@H](C(C)(C)O1)CC2=C1C=C(O3)C(C=CC3=O)=C2)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (152.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0454 mL 15.2272 mL 30.4544 mL
5 mM 0.6091 mL 3.0454 mL 6.0909 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.99%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0454 mL 15.2272 mL 30.4544 mL 76.1360 mL
5 mM 0.6091 mL 3.0454 mL 6.0909 mL 15.2272 mL
10 mM 0.3045 mL 1.5227 mL 3.0454 mL 7.6136 mL
15 mM 0.2030 mL 1.0151 mL 2.0303 mL 5.0757 mL
20 mM 0.1523 mL 0.7614 mL 1.5227 mL 3.8068 mL
25 mM 0.1218 mL 0.6091 mL 1.2182 mL 3.0454 mL
30 mM 0.1015 mL 0.5076 mL 1.0151 mL 2.5379 mL
40 mM 0.0761 mL 0.3807 mL 0.7614 mL 1.9034 mL
50 mM 0.0609 mL 0.3045 mL 0.6091 mL 1.5227 mL
60 mM 0.0508 mL 0.2538 mL 0.5076 mL 1.2689 mL
80 mM 0.0381 mL 0.1903 mL 0.3807 mL 0.9517 mL
100 mM 0.0305 mL 0.1523 mL 0.3045 mL 0.7614 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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