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  2. Kisspeptin-10 Trifluoroacetate

Kisspeptin-10 Trifluoroacetate 

Cat. No.: HY-P0254A Purity: 99.62%
Handling Instructions

Kisspeptin-10 Trifluoroacetate is the trifluoroacetate salt form of Kisspeptin-10. Kisspeptin-10, the minimal kisspeptin sequence with biological activity, is a potent endogenous ligand for GPR54.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Kisspeptin-10 Trifluoroacetate Chemical Structure

Kisspeptin-10 Trifluoroacetate Chemical Structure

Size Price Stock Quantity
1 mg USD 90 In-stock
Estimated Time of Arrival: December 31
5 mg USD 360 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Kisspeptin-10 Trifluoroacetate:

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Kisspeptin-10 Trifluoroacetate is the trifluoroacetate salt form of Kisspeptin-10. Kisspeptin-10, the minimal kisspeptin sequence with biological activity, is a potent endogenous ligand for GPR54.

IC50 & Target

Target: GPR54[1][2]

In Vitro

Kisspeptin-10 is the minimal kisspeptin sequence with full intrinsic bioactivity. Kisspeptins are a family of peptides encoded by the Kiss1 gene and are the natural ligands of the orphan g protein-coupled receptor GPR54[1]. Kisspeptins act as the principal positive regulator of the reproductive axis by directly stimulating gonadotropin-releasing hormone (GnRH) neuron activity. Kisspeptin-10 also has a direct stimulating effect on luteinizing hormone (LH) secretion in bovine anterior pituitary (AP) cells[2]. Different concentrations of Kisspeptin-10 have distinct effects on migration and proliferation of endothelial cells. Migration and proliferation of endothelial cells are increased at lower concentration of Kisspeptin-10 specially at 100 nM while higher concentration reduced both migration and proliferation[3].

In Vivo

Intravenous infusion of kisspeptin-10 (7.5, 35, and 100 nM) induces a dose-dependent increase in LH secretion. The stimulatory effect of kisspeptin-10 (100 n nM) on LH secretion is blocked by the GnRH antagonist cetrorelix, precluding a singular action on gonadotropes[4]. Kisspeptin-10 inhibits angiogenesis in vivo. Kp-10 inhibits tumor growth in SCID mice xenografted with human prostate cancer cells (PC-3) through inhibiting tumor angiogenesis[5].

Clinical Trial
Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 4 mg/mL (2.82 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7060 mL 3.5299 mL 7.0599 mL
5 mM --- --- ---
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[3]

HUVEC proliferation is measured by MTT Cell Proliferation Assay. Briefly, 5000 Human Umbilical Vein Endothelial cells/well are seeded in a 96-well plate and after the cells attached, they are treated with 1-100 or 500 nM kisspeptin-10 for 48 hours and one other group of cells is not treated. Then MTT reagent added to the wells and after incubation for 4 hours, the absorbance each well measured at 570 nm. Experiments are performed in triplicate[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4][5]

Rats[4]

Each female Sprague Dawley rat receives a single dose of 7.5, 35, or 100 nM kisspeptin-10 in 0.3 mL saline (n=6-8 per treatment group). Control rats receive 0.3 mL saline iv (n=5). In a separate group of rats, the selective GnRH antagonist cetrorelix (35 nM in 0.3 mL saline, iv; n=8) is administered after a control period of 90 min of blood sampling. Thirty minutes later, the rats are administered kisspeptin-10 (100 nM, iv), and automated blood sampling continued for another 4 h[4].

Mice[5]

3×106PC-3 human prostate tumor cells are implanted subcutaneously on the back of 4-week-old male SCID mice. After tumor volume reaching 50mm3, kisspeptin-10 (50 μg/50 μL/mouse) is injected intralesionally every day. Control groups are injected only with PBS. The growth of the tumor xenograft is evaluated in a pilot study by determining the tumor volume using digital caliper every 2 days. Tumor growth is measured. Mice are continually observed until are killed[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1416.46

Formula

C₆₃H₈₃N₁₇O₁₄.C₂HF₃O₂

Sequence

Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2

Sequence Shortening

YNWNSFGLRF-NH2

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Kisspeptin-10 Trifluoroacetate
Cat. No.:
HY-P0254A
Quantity:

Kisspeptin-10 Trifluoroacetate

Cat. No.: HY-P0254A