1. Peptides

Kisspeptin-10 Trifluoroacetate 

Cat. No.: HY-P0254A Purity: 99.62%
Handling Instructions

Kisspeptin-10 Trifluoroacetate is the trifluoroacetate salt form of Kisspeptin-10. Kisspeptin-10, the minimal kisspeptin Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2;YNWNSFGLRF-NH2.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Kisspeptin-10 Trifluoroacetate Chemical Structure

Kisspeptin-10 Trifluoroacetate Chemical Structure

Size Price Stock Quantity
1 mg USD 90 In-stock
Estimated Time of Arrival: December 31
5 mg USD 360 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Other Forms of Kisspeptin-10 Trifluoroacetate:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Kisspeptin-10 Trifluoroacetate is the trifluoroacetate salt form of Kisspeptin-10. Kisspeptin-10, the minimal kisspeptin Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2;YNWNSFGLRF-NH2.

IC50 & Target

Target: GPR54[1][2]

In Vitro

Kisspeptin-10 is the minimal kisspeptin sequence with full intrinsic bioactivity. Kisspeptins are a family of peptides encoded by the Kiss1 gene and are the natural ligands of the orphan g protein-coupled receptor GPR54[1]. Kisspeptins act as the principal positive regulator of the reproductive axis by directly stimulating gonadotropin-releasing hormone (GnRH) neuron activity. Kisspeptin-10 also has a direct stimulating effect on luteinizing hormone (LH) secretion in bovine anterior pituitary (AP) cells[2]. Different concentrations of Kisspeptin-10 have distinct effects on migration and proliferation of endothelial cells. Migration and proliferation of endothelial cells are increased at lower concentration of Kisspeptin-10 specially at 100 nM while higher concentration reduced both migration and proliferation[3].

In Vivo

Intravenous infusion of kisspeptin-10 (7.5, 35, and 100 nM) induces a dose-dependent increase in LH secretion. The stimulatory effect of kisspeptin-10 (100 n nM) on LH secretion is blocked by the GnRH antagonist cetrorelix, precluding a singular action on gonadotropes[4]. Kisspeptin-10 inhibits angiogenesis in vivo. Kp-10 inhibits tumor growth in SCID mice xenografted with human prostate cancer cells (PC-3) through inhibiting tumor angiogenesis[5].

Clinical Trial
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
Cell Assay
[3]

HUVEC proliferation is measured by MTT Cell Proliferation Assay. Briefly, 5000 Human Umbilical Vein Endothelial cells/well are seeded in a 96-well plate and after the cells attached, they are treated with 1-100 or 500 nM kisspeptin-10 for 48 hours and one other group of cells is not treated. Then MTT reagent added to the wells and after incubation for 4 hours, the absorbance each well measured at 570 nm. Experiments are performed in triplicate[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4][5]

Rats[4]

Each female Sprague Dawley rat receives a single dose of 7.5, 35, or 100 nM kisspeptin-10 in 0.3 mL saline (n=6-8 per treatment group). Control rats receive 0.3 mL saline iv (n=5). In a separate group of rats, the selective GnRH antagonist cetrorelix (35 nM in 0.3 mL saline, iv; n=8) is administered after a control period of 90 min of blood sampling. Thirty minutes later, the rats are administered kisspeptin-10 (100 nM, iv), and automated blood sampling continued for another 4 h[4].

Mice[5]

3×106PC-3 human prostate tumor cells are implanted subcutaneously on the back of 4-week-old male SCID mice. After tumor volume reaching 50mm3, kisspeptin-10 (50 μg/50 μL/mouse) is injected intralesionally every day. Control groups are injected only with PBS. The growth of the tumor xenograft is evaluated in a pilot study by determining the tumor volume using digital caliper every 2 days. Tumor growth is measured. Mice are continually observed until are killed[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1416.46

Formula

C₆₃H₈₃N₁₇O₁₄.C₂HF₃O₂

Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Kisspeptin-10 Trifluoroacetate
Cat. No.:
HY-P0254A
Quantity:

Kisspeptin-10 Trifluoroacetate

Cat. No.: HY-P0254A