1. Apoptosis
  2. MDM-2/p53
  3. PhiKan 083 hydrochloride

PhiKan 083 hydrochloride 

Cat. No.: HY-108637A Purity: >99.0%
Handling Instructions

PhiKan 083 hydrochloride is a carbazole derivative, which binds to the surface cavity and stabilizes Y220C (a p53 mutant), with a Kd of 167 μM, and a relative binding affinity (Kd) of 150 μM in Ln229 cells.

For research use only. We do not sell to patients.

PhiKan 083 hydrochloride Chemical Structure

PhiKan 083 hydrochloride Chemical Structure

CAS No. : 1050480-30-2

Size Price Stock Quantity
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 140 In-stock
Estimated Time of Arrival: December 31
25 mg USD 300 Get quote
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Other Forms of PhiKan 083 hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

PhiKan 083 hydrochloride is a carbazole derivative, which binds to the surface cavity and stabilizes Y220C (a p53 mutant), with a Kd of 167 μM[1], and a relative binding affinity (Kd) of 150 μM in Ln229 cells[3].

IC50 & Target

Kd: 167 μM (p53-Y220C)[1], 150 μM (p53Y220C, in Ln229 cells)[3]

In Vitro

PhiKan 083 is a carbazole derivative, which binds to the surface cavity and stabilizes Y220C (a p53 mutant), with a Kd of 167 μM[1], shows a relative binding affinity (Kd) of 150 μM for p53Y220C in Ln229 cells[3].
PhiKan 083 slows down its thermal denaturation rate[2].
PhiKan 083 (125 μM, 48 hours) reduces the cell viability of engineered variants of Ln229 cells[3].
PhiKan 083 (100 μM) in conbination with doxorubicin (1 μM) enhances the pro-apoptotic activity in all variants of Ln229 cells (p53wt, p53Y220C, p53G245S, p53R282W)[3].

Cell Viability Assay[1]

Cell Line: Ln229, Ln229-p53-wt, Ln229-p53-Y220C, Ln229-p53-G245S, Ln229-p53-R282W cells
Concentration: 125 μM
Incubation Time: 48 hours
Result: Caused ∼70 ± 5% reduction in cell viability of variants of Ln229 cells.
Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 2 mg/mL (7.28 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6391 mL 18.1957 mL 36.3914 mL
5 mM 0.7278 mL 3.6391 mL 7.2783 mL
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

274.79

Formula

C₁₆H₁₉ClN₂

CAS No.

1050480-30-2

SMILES

CNCC1=CC2=C(C=C1)N(CC)C3=C2C=CC=C3.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere

Purity: >99.0%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PhiKan 083 hydrochloride
Cat. No.:
HY-108637A
Quantity:

PhiKan 083 hydrochloride

Cat. No.: HY-108637A