1. Apoptosis
  2. MDM-2/p53
  3. NSC59984

NSC59984 

Cat. No.: HY-19726 Purity: 99.97%
Handling Instructions

NSC59984 induces mutant p53 protein degradation via MDM2 and the ubiquitin-proteasome pathway. NSC59984 acts by targeting GOF-mutant p53 and stimulates p73 to restore the p53 pathway signaling.

For research use only. We do not sell to patients.

NSC59984 Chemical Structure

NSC59984 Chemical Structure

CAS No. : 803647-40-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 158 In-stock
Estimated Time of Arrival: December 31
5 mg USD 144 In-stock
Estimated Time of Arrival: December 31
10 mg USD 240 In-stock
Estimated Time of Arrival: December 31
50 mg USD 960 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1680 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

NSC59984 induces mutant p53 protein degradation via MDM2 and the ubiquitin-proteasome pathway[1]. NSC59984 acts by targeting GOF-mutant p53 and stimulates p73 to restore the p53 pathway signaling[2].

In Vitro

NSC59984 specifically restores p53 pathway signaling in mutant p53-expressing human colorectal cancer cells. NSC59984 induces cell death in tumor cells but not normal cells with little or no genotoxicity. NSC59984 induces mutant p53 protein degradation through MDM2-mediated ubiquitination in cancer cells. NSC59984 restores p53 pathway signaling through activation of p73. NSC59984 induces p73-dependent cell apoptosis in cancer. The EC50 of NSC59984 in most cancer cells is significantly lower than those of normal cells, with EC50 of 8.38 μM for p53-null HCT116 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NSC59984 synergizes with CPT11 to induce cell death in mutant p53-expressing colorectal cancer cells and inhibits mutant p53-associated colon tumor xenograft growth in a p73-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

265.27

Formula

C₁₂H₁₅N₃O₄

CAS No.
SMILES

O=C(N1CCN(C)CC1)/C=C/C2=CC=C([N+]([O-])=O)O2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (125.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7697 mL 18.8487 mL 37.6974 mL
5 mM 0.7539 mL 3.7697 mL 7.5395 mL
10 mM 0.3770 mL 1.8849 mL 3.7697 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.97%

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Keywords:

NSC59984NSC 59984NSC-59984MDM-2/p53Inhibitorinhibitorinhibit

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Product Name:
NSC59984
Cat. No.:
HY-19726
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