1. Apoptosis
  2. MDM-2/p53

DPBQ 

Cat. No.: HY-U00441
Handling Instructions

DPBQ is a p53 activator.

For research use only. We do not sell to patients.

DPBQ Chemical Structure

DPBQ Chemical Structure

CAS No. : 7029-89-2

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10 mg USD 290 Get quote
25 mg USD 650 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

DPBQ is a p53 activator.

IC50 & Target

p53[1]

In Vitro

DPBQ elicits apoptosis and cell death and is selective for effects in 4N cells. DPBQ elicits expression and phosphorylation of p53 and this effect is specific to tetraploid cells. Knockdown of TP53 restores proliferation of tetraploid cells in the presence of DPBQ[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7595 mL 13.7977 mL 27.5953 mL
5 mM 0.5519 mL 2.7595 mL 5.5191 mL
10 mM 0.2760 mL 1.3798 mL 2.7595 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

For Western blot analysis, 2N and 4N RPE1 cells are treated with DMSO, 50 ng/mL doxorubicin, or 1 μM DPBQ for 48 hours. Western blotting is performed with a SDS-PAGE Electrophoresis and semidry transfer. Samples are analyzed by chemiluminescence. β-actin is used as a protein loading control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

For Apoptosis assays, 2N and 4N RPE1 cells are treated with DMSO, 60 nM staurosporine, or 1 μM DPBQ for 48 hours. Apoptosis is evaluated by an Annexin V-PE/7-AAD apoptosis detection kit. For the flow cytometry analysis, the percent positive cells in the upper right and lower right quadrants are summed to give the total number of apoptotic cells. Three independent replicates are performed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

362.38

Formula

C₂₄H₁₄N₂O₂

CAS No.

7029-89-2

SMILES

O=C(C1=CC=CC=C12)C3=C(N=C(C4=CC=CC=C4)C(C5=CC=CC=C5)=N3)C2=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
DPBQ
Cat. No.:
HY-U00441
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DPBQ

Cat. No.: HY-U00441