Amifostine thiol
Based on 1 publication(s) in Google Scholar
Amifostine thiol (WR-1065) is an active metabolite of the cytoprotector Amifostine (HY-B0639). Amifostine thiol is a cytoprotective agent with radioprotective abilities. Amifostine thiol activates p53 through a JNK-dependent signaling pathway.
For research use only. We do not sell to patients.
- Purity: 98.49%
- CAS No.: 31098-42-7
- Formula: C5H14N2S
- Molecular Weight:134.24
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Amifostine thiol
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Biological Activity
p53[2]
Amifostine thiol can reduce both the direct and delayed detrimental effects of both high and low-LET radiation exposures[1].
Amifostine thiol attenuates both the DNA damage and the G1-phase arrest induced by radiation[1].
Amifostine thiol (4 mM; 30 minutes) protects RKO36 cells from chromosomal damage and death induced by ionizing radiation[1].
Amifostine thiol (4 mM; 30 minutes) protects irradiated RKO36 cells from delayed genomic instability[1].
Amifostine thiol is cytotoxic to RKO36 cells at milimolar concentrations, especially after continuous treatment[1].
Amifostine thiol at 40 μM protects RKO36 cells from delayed genomic instability but not from cell death and immediate chromosomal damage[1].
Amifostine thiol activates JNK resulting in the phosphorylation of p53 at threonine 81[2].
Amifostine thiol affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 31098-42-7
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Appearance Solid
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Molecular Weight 134.24
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Formula C5H14N2S
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Color White to off-white
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SMILES
NCCCNCCS
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Synonyms
WR-1065
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Front Cell Dev Biol
2020 Jul 29;8:703. PMID: 32850823
Solvent & Solubility
H2O : 250 mg/mL (1862.34 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Jaroslaw Dziegielewski, et al. WR-1065, the Active Metabolite of Amifostine, Mitigates Radiation-Induced Delayed Genomic Instability. Free Radic Biol Med. 2008 Dec 15; 45(12): 1674-1681. [Content Brief]
[2]. Olivier Pluquet, et al. The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J Biol Chem. 2003 Apr 4;278(14):11879-87. J Biol Chem. 2003 Apr 4;278(14):11879-87. [Content Brief]
[3]. J. S. Murley, et al. WR-1065, an active metabolite of the cytoprotector amifostine, affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells. Cell Prolif. 1997 Jun; 30(6): 283-294. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.4493 mL | 37.2467 mL | 74.4934 mL | 186.2336 mL |
| 5 mM | 1.4899 mL | 7.4493 mL | 14.8987 mL | 37.2467 mL | |
| 10 mM | 0.7449 mL | 3.7247 mL | 7.4493 mL | 18.6234 mL | |
| 15 mM | 0.4966 mL | 2.4831 mL | 4.9662 mL | 12.4156 mL | |
| 20 mM | 0.3725 mL | 1.8623 mL | 3.7247 mL | 9.3117 mL | |
| 25 mM | 0.2980 mL | 1.4899 mL | 2.9797 mL | 7.4493 mL | |
| 30 mM | 0.2483 mL | 1.2416 mL | 2.4831 mL | 6.2078 mL | |
| 40 mM | 0.1862 mL | 0.9312 mL | 1.8623 mL | 4.6558 mL | |
| 50 mM | 0.1490 mL | 0.7449 mL | 1.4899 mL | 3.7247 mL | |
| 60 mM | 0.1242 mL | 0.6208 mL | 1.2416 mL | 3.1039 mL | |
| 80 mM | 0.0931 mL | 0.4656 mL | 0.9312 mL | 2.3279 mL | |
| 100 mM | 0.0745 mL | 0.3725 mL | 0.7449 mL | 1.8623 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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