2. Apoptosis
  3. MDM-2/p53
  4. Amifostine thiol

Amifostine thiol  (Synonyms: WR-1065)

Cat. No.: HY-137864 Purity: ≥95.0%
COA Handling Instructions

Molarity Calculator

Amifostine thiol (WR-1065) is an active metabolite of the cytoprotector Amifostine (HY-B0639). Amifostine thiol is a cytoprotective agent with radioprotective abilities. Amifostine thiol activates p53 through a JNK-dependent signaling pathway.

For research use only. We do not sell to patients.

Amifostine thiol Chemical Structure

Amifostine thiol Chemical Structure

CAS No. : 31098-42-7

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5 mg USD 58 In-stock
10 mg USD 90 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Amifostine thiol:

Amifostine thiol Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Amifostine thiol

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Amifostine thiol (WR-1065) is an active metabolite of the cytoprotector Amifostine (HY-B0639). Amifostine thiol is a cytoprotective agent with radioprotective abilities. Amifostine thiol activates p53 through a JNK-dependent signaling pathway[1][2][3].

IC50 & Target

p53[2]
In Vitro

Amifostine thiol can reduce both the direct and delayed detrimental effects of both high and low-LET radiation exposures[1].
Amifostine thiol attenuates both the DNA damage and the G1-phase arrest induced by radiation[1].
Amifostine thiol (4 mM; 30 minutes) protects RKO36 cells from chromosomal damage and death induced by ionizing radiation[1].
Amifostine thiol (4 mM; 30 minutes) protects irradiated RKO36 cells from delayed genomic instability[1].
Amifostine thiol is cytotoxic to RKO36 cells at milimolar concentrations, especially after continuous treatment[1].
Amifostine thiol at 40 μM protects RKO36 cells from delayed genomic instability but not from cell death and immediate chromosomal damage[1].
Amifostine thiol activates JNK resulting in the phosphorylation of p53 at threonine 81[2].
Amifostine thiol affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Amifostine thiol Related Antibodies
Molecular Weight

134.24

Formula

C5H14N2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NCCCNCCS
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1862.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (744.93 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.4493 mL 37.2467 mL 74.4934 mL
5 mM 1.4899 mL 7.4493 mL 14.8987 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 16.67 mg/mL (124.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥95.0%

COA (240 KB) HNMR (228 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 7.4493 mL 37.2467 mL 74.4934 mL 186.2336 mL
5 mM 1.4899 mL 7.4493 mL 14.8987 mL 37.2467 mL
10 mM 0.7449 mL 3.7247 mL 7.4493 mL 18.6234 mL
15 mM 0.4966 mL 2.4831 mL 4.9662 mL 12.4156 mL
20 mM 0.3725 mL 1.8623 mL 3.7247 mL 9.3117 mL
25 mM 0.2980 mL 1.4899 mL 2.9797 mL 7.4493 mL
30 mM 0.2483 mL 1.2416 mL 2.4831 mL 6.2078 mL
40 mM 0.1862 mL 0.9312 mL 1.8623 mL 4.6558 mL
50 mM 0.1490 mL 0.7449 mL 1.4899 mL 3.7247 mL
60 mM 0.1242 mL 0.6208 mL 1.2416 mL 3.1039 mL
80 mM 0.0931 mL 0.4656 mL 0.9312 mL 2.3279 mL
100 mM 0.0745 mL 0.3725 mL 0.7449 mL 1.8623 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Amifostine thiol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amifostine thiol31098-42-7WR-1065WR1065WR 1065MDM-2/p53genomicinstabilityamifostineradiationdelayed effectsradioprotectioncytoprotectiveInhibitorinhibitorinhibit

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