2. Apoptosis Epigenetics Cell Cycle/DNA Damage
  3. Apoptosis Caspase PARP
  4. AC-45594

AC-45594  (Synonyms: 4-(Hexyloxy)phenol)

Cat. No.: HY-W009929 Purity: 99.94%
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AC-45594 (4-(Hexyloxy)phenol) is a UPR activator. AC-45594 induces endoplasmic reticulum stress, activates the unfolded protein response (UPR), and drives the transition from adaptive stress signaling to terminal stress signaling, ultimately leading to cell Apoptosis. AC-45594 activates Caspase-3, induces PARP cleavage, and increases the protein level of DR5. AC-45594 selectively inhibits the growth of Ewing sarcoma cells.

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AC-45594

AC-45594 Chemical Structure

CAS No. : 18979-55-0

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AC-45594 Related Antibodies

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

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Description

AC-45594 (4-(Hexyloxy)phenol) is a UPR activator. AC-45594 induces endoplasmic reticulum stress, activates the unfolded protein response (UPR), and drives the transition from adaptive stress signaling to terminal stress signaling, ultimately leading to cell Apoptosis. AC-45594 activates Caspase-3, induces PARP cleavage, and increases the protein level of DR5. AC-45594 selectively inhibits the growth of Ewing sarcoma cells[1].

IC50 & Target[1]

Caspase 3

 

Caspase 8

 

Caspase-7

 

In Vitro

AC-45594 (72 h) selectively inhibits the growth of Ewing sarcoma cell lines COG-E-352, SK-ES-1, TC-32, SKNMC, TC-71 and TC-106 in a concentration-dependent manner, with EC50 values ranging from 45.0 ± 2.2 to 144.3 ± 13.0 nM[1].
AC-45594 (25-200 nM; 72 h) induces apoptosis in SK-ES-1 and TC-71 Ewing sarcoma cell lines, while reducing the cell populations in the S and G2/M phases[1].
AC-45594 (200 nM; 8-16 h) triggers sequential activation of the unfolded protein response and pro-apoptotic signaling in SK-ES-1 Ewing sarcoma cells, with early stress response changes observed at 8 h post-treatment, and extensive UPR activation and apoptosis initiation detected at 16 h post-treatment[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AC-45594 Related Antibodies

Cell Cycle Analysis[1]

Cell Line: Ewing sarcoma cell lines (SK-ES-1, TC-71)
Concentration: 25, 50, 100 nM
Incubation Time: 72 h
Result: Did not alter cell cycle distribution in SK-ES-1 or TC-71 cells at 50 nM (below EC50 values for both cell lines).
Significantly reduced the proportion of SK-ES-1 cells in S (12.97 ± 0.32% vs 21.83 ± 0.49% in control) and G2/M (20.27 ± 0.03% vs 22.57 ± 0.15% in control) phases, and increased apoptotic cells to 4.70 ± 0.12% at 100 nM.
Reduced the proportion of TC-71 cells in S (12.97 ± 0.32% vs 20.60 ± 0.90% in control) and G2/M (16.20 ± 0.38% vs 25.47 ± 0.27% in control) phases, and increased apoptotic cells to 20.23 ± 0.20% at 100 nM.

Western Blot Analysis[1]

Cell Line: Ewing sarcoma cell lines (SK-ES-1, TC-71)
Concentration: 100, 200, 300 nM (72 h); 200 nM (8, 16, 24, 48 h)
Incubation Time: 8, 16, 24, 48, 72 h
Result: Activated caspase-3 and induced PARP cleavage in TC-71 and SK-ES-1 cells at 100 nM or higher.
Activated caspase-3, -7, and -8, induced PARP cleavage, and increased DR5 protein levels as early as 8 hours in both cell lines at 200 nM.
Molecular Weight

194.27

Formula

C12H18O2
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

CCCCCCOC1=CC=C(O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, stored under nitrogen

In solvent -80°C 1 year
-20°C 6 months
Purity & Documentation

Purity: 99.94%

SDS (476 KB)

COA (242 KB) HNMR (118 KB) GC (178 KB)

Handling Instructions (2659 KB)
References
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AC-45594 Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AC-4559418979-55-04-(Hexyloxy)phenolAC45594AC 45594ApoptosisCaspasePARPpoly ADP ribose polymeraseSK-ES-1extrinsic apoptotic pathwaysTC-32Ewing sarcoma cellsTC-71endoplasmic reticulum stressCOG-E-352NR5A1/SF-1intrinsic apoptotic pathwaysunfolded protein responseInhibitorinhibitorinhibit

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