ALK-IN-34
ALK-IN-34 is an Anaplastic Lymphoma Kinase (ALK) inhibitor with an IC50 of 0.26 μM. ALK-IN-34 demonstrates antiproliferative activity against lung cancer cells and gastric adenocarcinoma cells.
For research use only. We do not sell to patients.
- Formula: C25H21N3O
- Molecular Weight:379.45
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
ALK-IN-34 (compound 8c) (1-100 μM; 72 h) potently inhibits proliferation of Karpas 299 cells (IC50 = 2.17 μM) and H2228 cells (IC50 = 6.02 μM), with lower potency against A549 and AGS cells[1].
ALK-IN-34 (10 μM) shows greater selectivity towards ALK but also significantly inhibits EGFR and VEGFR2 kinases at 10 μM[1].
ALK-IN-34 has lower toxicity towards L929 normal cells (IC50 = 102.2 μM) compared to Karpas 299 cancer cells, with a selectivity index of 47.09[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Karpas 299 (NPM-ALK-positive ALCL), H2228 (EML4-ALK-positive NSCLC), A549 (ALK-negative NSCLC), AGS (gastric adenocarcinoma)
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Concentration:1, 5, 10, 25, 50, 100 μM
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Incubation Time:72 h
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Result:Inhibited proliferation of Karpas 299 cells with an IC₅₀ of 2.17 μM, H2228 cells with an IC₅₀ of 6.02 μM, A549 cells with an IC₅₀ of 41 μM, and AGS cells with an IC₅₀ of 45.8 μM.
Chemical Information
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Molecular Weight 379.45
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Formula C25H21N3O
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SMILES
COC(C=C1)=CC=C1C2=C3C(C4=CC=CC=C4CC3)=NC(NC5=CC=CC=C5)=N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ALK-IN-34 Related Classifications
HY-181113 Related Classifications
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