Alkaline Phosphatase (ALP), Porcine Kidney
Based on 1 Customer Validation
Alkaline Phosphatase (ALP), Porcine Kidney (ALP, Orthophosphoric-Monoester Phosphohydrolase) is a biological material or organic compound that can be used in life science research.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
EC Number
EC 3.1.3.1
Chemical Information
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SMILES
[Alkaline Phosphatase (ALP), Porcine Kidney]
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Synonyms
ALP, Orthophosphoric-Monoester Phosphohydrolase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
Purity & Documentation
References
[1]. Alkaline Phosphatase (ALP), Porcine Kidney [Content Brief]
[2]. Nicolson GL, Singer SJ. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes. Proc Natl Acad Sci U S A. 1971 May;68(5):942-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Alkaline Phosphatase (ALP), Porcine Kidney Related Classifications
HY-E70359 Related Classifications
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