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United States

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Cat. No.: HY-W591975

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Alkyne maleimide

Alkyne maleimide is a bifunctional linker reagent with a terminal malimide group and a propargyl group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry.

For research use only. We do not sell to patients.

CAS No.: 2785323-77-3
Formula: C₁₂H₁₄N₂O₃
Molecular Weight:234.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (239 KB)

Alkyne maleimide Chemical Structure

CAS No.: 2785323-77-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-138246
Pentynoic acid STP ester sodium

Click Chemistry Reagent

Biological Activity

Description

Chemical Information

CAS No. 2785323-77-3
Molecular Weight 234.25
Formula C₁₂H₁₄N₂O₃
SMILES

C#CCCCC(NCCN1C(C=CC1=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (239 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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