AM2-10

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.1 Solvent: OptiMEM.
1.2 Concentration recommendation: 20 μM.

3. Staining Procedure
3.1 Sample type: Adherent cells (PC12 cells grown on poly-L-lysine coverslips).[1]
3.2 Incubation conditions: Incubate cells with 20 μM AM2-10 for 5 min at 37°C; or image cells in solution containing AM2-10 for NGF-induced differentiation assessment.
3.3 Washing steps: Wash cells twice with ice-cold PBS.

5. Detection & Analysis
5.1 Instrument: Fluorescence microscope.
5.2 Result analysis:
5.2.1 Fluorescence localization: Stains all cellular membranes, including endocytic vesicles and neurite membranes.
5.2.2 Result application: Used to assess fluid-phase endocytosis efficiency and NGF-induced neurite differentiation in PC12 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.