Anti-Mouse IL-12 p40 Antibody (C17.8)
Based on 1 Customer Validation
Anti-Mouse IL-12 p40 Antibody (C17.8) is a rat-derived anti-mouse IL-12 p40 IgG2a κ type antibody inhibitor. Anti-Mouse IL-12 p40 Antibody (C17.8) binds p40 subunit of IL-12. Anti-Mouse IL-12 p40 Antibody (C17.8) can be used for the researches of inflammation, immunology and metabolic disease, such as diabetes and arthritis.
For research use only. We do not sell to patients.
- Purity : 97.691%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
IL-12 p40
In Vitro
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse IL-12 p40 Antibody (C17.8) (200 μg, i.p., for 6 consecutive days) results in the enhancement of diabetes in 2-week-old non-obese diabetic mice models[1].
Anti-Mouse IL-12 p40 Antibody (C17.8) (0.5 mg, i.p., 2 h before injection of SCW fragments) suppresses murine streptococcal cell wall (SCW)-induced arthritis[2].
Anti-Mouse IL-12 p40 Antibody (C17.8) (0.5 mg, i.p., once a week) prolongs survival in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis mice models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Non-obese diabetic mice models[1]
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Dosage:500 μg
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Administration:Intraperitoneally injection, twice a week during 5-30 weeks
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Result:Completely prevented diabetes.
Significantly decreased the degree of insulitis.
Only 62% of NOD mice at 15 weeks of age.
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Animal Model:2-week-old non-obese diabetic mice models[1]
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Dosage:200 μg
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Administration:Intraperitoneally injection, for 6 consecutive days
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Result:Only 56% of NOD mice at 10 weeks of age.
Inhibited IL-2 and IFN-γ production at 4 weeks.
Enhanced production of IL-2 and IFN-γ and suppressed production of IL-4 and IL-10 at 8 weeks of age.
Increased CD25+ and CD44high cells in both CD4+ and CD8+ subsets at 8 days after the final injection.
Resulted in a rapid onset of overt diabetes when Splenic T-cells transferred to NOD-scid with spleen cells from diabetic NOD mice.
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Animal Model:Streptococcal cell wall (SCW)-induced arthritis mice models[2]
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Dosage:0.5 mg
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Administration:Intraperitoneally injection, 2 hours before injection of SCW fragments
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Result:Increased the chondrocyte PG synthesis.
Reduced infiltration of synovial membrane with inflammatory cells.
Decreased mRNA levels for IL1β, IL12 and TNFα and increased IL1Ra and IL10 mRNA levels.
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Animal Model:B6D2F1 mice were injected with 80 million B6 spleen cells[3]
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Dosage:0.5 mg
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Administration:Intraperitoneally injection, every 7 days subsequently
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Result:Significantly prolonged survival of mice.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo IL-12p40 neutralization; p40 affinity chromatography; Immunoprecipitation; ELISA; Flow c
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse IL-12 p40 Antibody (C17.8)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Fujihira K, et al. Suppression and acceleration of autoimmune diabetes by neutralization of endogenous interleukin-12 in NOD mice. Diabetes. 2000 Dec;49(12):1998-2006. [Content Brief]
[2]. Joosten LA, et al. Blockade of endogenous interleukin 12 results in suppression of murine streptococcal cell wall arthritis by enhancement of interleukin 10 and interleukin 1Ra. Ann Rheum Dis. 2000 Mar;59(3):196-205. [Content Brief]
[3]. Gaignage M, et al. Novel antibodies that selectively block mouse IL-12 enable the re-evaluation of the role of IL-12 in immune protection and pathology. Eur J Immunol. 2021 Jun;51(6):1482-1493. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse IL-12 p40 Antibody (C17.8) Related Classifications
HY-P990225 Related Classifications
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