Antibacterial agent 336 

Antibacterial agent 336 is an antibacterical agent with broad-spectrum inhibitory activity against Gram-negative and Gram-positive bacteria. Antibacterial agent 336 binds tightly via its pyridine group to the carboxyl group, Lys-465 residue, and Leu-480 residue of the MsbA protein, interfering with bacterial outer membrane synthesis, and exerts concentration-dependent bactericidal activity against bacterial. Antibacterial agent 336 has low cytotoxicity to normal human liver cells. Antibacterial agent 336 can be used for the research of bacterial infections^[1].

Antibacterial agent 336 (Compound z10) exhibits broad-spectrum in vitro antibacterial activity against E. coli ATCC 25922, Salmonella ATCC 12022, S. aureus ATCC 25922, and P. aeruginosa ATCC 27853 with MIC values of 1.40, 1.40, 1.75, and 2.33 μM, respectively^[1].
Antibacterial agent 336 exhibits low in vitro cytotoxicity toward normal human liver L02 cells, with an IC₅₀ of 378.6 μM and a selectivity index of 216.3^[1].
Antibacterial agent 336 (0.5×MIC-2×MIC; 2-25 h) exerts a concentration-dependent bactericidal effect against E. coli ATCC 25922, P. aeruginosa ATCC 27853, Salmonella ATCC 12022, and S. aureus ATCC 25922, with significant bacterial kill observed within 2 h at 1×MIC and 2×MIC concentrations^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

428.55

C₂₂H₂₈N₄O₃S

CCC1CCC(C(C(O)=O)=C(NC(CN2CCN(C3=NC=CC=C3)CC2)=O)S4)=C4C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sun C, et al. Discovery of novel thiophene amide derivatives with potent antibacterial activity. Bioorg Med Chem Lett. Published online March 25, 2026.