2B4 Antibody (YA1382)

Cat. No.: HY-P81637: Ficha de datos COA
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2B4 Antibody (YA1382) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to 2B4.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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2B4 Antibody (YA1382) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

2B4 Antibody (YA1382) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to 2B4.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BZW8

Gene ID

51744 [NCBI]

Immunogen

Purified recombinant fragment of human CD244 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Pureza	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:11714776, PubMed:8376943). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:11714776, PubMed:8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity)

Subcellular Localization：Membrane; Single-pass type I membrane protein; Cell membrane; Membrane raft

Expression：
Tissue_specificity:It is expressed in the spleen and peripheral blood lymphocytes, followed by the lungs, liver, testes, and small intestine. It is also expressed on all natural killer (NK) cells, monocytes and basophils, TCR-γ/δ+ T cells, monocytes, basophils, and some CD8 (+) T cells.

Isoforms & Post-Translational Modification：Q9BZW8 has 4 isomers: Q9BZW8-1: 41616 Da (predicted); Q9BZW8-2: 41077 Da (predicted); Q9BZW8-3: 36987 Da (predicted); Q9BZW8-4: 30784 Da (predicted).
N-linked glycosylation is essential for the binding to its ligand CD48 (PubMed:21606496). Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding (PubMed:21606496);Phosphorylated by FYN and CSK on tyrosine residues following activation (PubMed:11034353). Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells (PubMed:10358138)

Subunit：Interacts with CD48 (PubMed:27249817, PubMed:9841922). Interacts (via phosphorylated ITSM 1-4) with SH2D1A (via SH2 domain); SH2D1A probably mediates association with FYN. Interacts (via phosphorylated ITSM 3) with PTPN11/SHP-2, INPP5D/SHIP1, PTPN6/SHP-1 and CSK; binding of SH2D1A/SAP prevents association with PTPN11, PTPN6 and CSK; conflictingly a similar association has been described for phosphorylated ITSM 1 also including GRB2 and PLCG1. Interacts weakly (via phosphorylated ITSM 2) with PTPN11/SHP-2 and CSK (PubMed:10358138, PubMed:10934222, PubMed:12458214, PubMed:15713798, PubMed:24642916, PubMed:26221972). Interacts with SH2D1B (PubMed:12458214, PubMed:24642916). Interacts with PIK3R1; PI3K recruits SH2D1A (PubMed:11815622). Interacts with MHC class I proteins; the interaction is proposed to prevent self-killing of NK cells

RRID

AB_3103746

Database

Entrez Gene: 51744 Human

SwissProt: Q9BZW8 Human

OMIM: 605554 Human

Research Field

Immunology

Synonyms

CD244; 2B4; Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NAIL; NK cell type I receptor protein 2B4; NKR2B4; h2B4; CD244

Documentación

Select Batch:

Ficha de datos (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

2B4 Antibody (YA1382) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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2B4 Antibody (YA1382)

2B4 Antibody (YA1382) Recomendaciones destacadas:

Product Detail

Background

Descripciòn

2B4 Antibody (YA1382) Related Classifications

Productos vistos recientemente:

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