AF700-conjugated CD274 (PD-L1) Antibody (29E.2A3)
(Synonyms: CD274 (PD-L1),CD274-AF700,29E.2A3)AF700-conjugated CD274 (PD-L1) Antibody (29E.2A3) is a Mouse-derived and AF700 conjugated monoclonal antibody, targeting to CD274 (PD-L1).
-
Host:
Mouse
-
Isotype:
Mouse IgG2b,κ
-
Application:
FC
-
Reactivity :
Human
-
Formulation:
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
-
Conjugation:
AF700
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
|
|---|---|
| Dilution Ratio | 5 ul per 106 cells in a 100 µl suspension |
Product Details
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
-
Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
-
Shipping
Shipping with blue ice.
Background
-
Function
CD274 (PD-L1) is an important immune checkpoint ligand protein belonging to the B7 family of immunoregulatory molecules. By binding to PD-1 on the surface of T cells, PD-L1 inhibits T-cell activation and immune responses; it is a key mechanism of tumor immune escape and a major target for current immune checkpoint therapies.
-
Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Single-pass type I membrane protein; Recycling endosome membrane; Single-pass type I membrane protein; Nucleus; Cell membrane; Single-pass type I membrane protein; Endomembrane system; Single-pass type I membrane protein; Secreted
-
Expression
Tissue_specificity:It is highly expressed in the heart, skeletal muscle, placenta, and lungs. It is weakly expressed in the thymus, spleen, kidneys, and liver. It is expressed on activated T cells and B cells, dendritic cells, keratinocytes, and monocytes; it is widely expressed, with the highest expression in the lungs, liver, pituitary gland, and various peripheral blood cells (including neutrophils and certain subtypes of lymphocytes and myeloid cells) .
Induction:Up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. Up-regulated in B-cells activated by surface Ig cross-linking -
Isoforms & Post-Translational Modification
CD274 (PD-L1) has 4 isomers: Q9NZQ7-1: 33275 Da (predicted); Q9NZQ7-2: 20246 Da (predicted); Q9NZQ7-3: 20454 Da (predicted); Q9NZQ7-4: 28050 Da (predicted).
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8; leading to degradation. Deubiquitinated by USP22; leading to stabilization -
Subunit
Interacts with PDCD1. Interacts (via transmembrane domain) with CMTM4 and CMTM6. Interacts with (phosphorylated) STAT3; promoting nuclear translocation. Interacts with CD80
-
Synonyms
CD274 (PD-L1),CD274-AF700,29E.2A3
Documentation
Powered by Bioz
